Expression and overproduction of recombinant penicillin G acylase from Kluyvera citrophila in Escherichia coli
文献类型：期刊论文
| 作者 | Wen, Y; Feng, MQ; Yuan, ZY; Zhou, P |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:2页码:233-237 |
| 关键词 | heterologous production Escherichia coli Kluyvera citrophila penicillin G acylase culture performance overproduction |
| 通讯作者 | Zhou, P (reprint author), Fudan Univ, Sch Pharm, Dept Drug Biosynth, Shanghai 200032, Peoples R China.,pzhou@shmu.edu.cn |
| 英文摘要 | Heterologous production of Kluyvera citrophila penicillin G acyalse (KcPGA) was optimized in Escherichia coli (E. coli). Several factors, including the effect of different medium and culture performance were identified to be crucial for the enzyme overproduction. The KcPGA yield was significantly and conveniently increased by more than 2.4-fold in TB medium compared to that in LB medium, which need inducing by isopropylthio-beta-D-galactoside (IPTG). The results shown the lower temperature (28 degrees C) was more feasible for the overproduction of KcPGA compared to the higher temperature (37 degrees C). The highest enzyme activity was obtained at 28 degrees C in TB medium only when culture pH was closed to its original pH value and the basic condition was more optimal for the production of KcPGA than that of acidic condition. On the other hand, the higher temperature (37 degrees C) culture condition resulted in poor enzyme activity although the optimum growth temperature for most of the E. coli strains is approximately 37 degrees C. It was possible due to the limitation by translation or posttranslational steps of the heterologous production of KcPGA in E. coli. The results suggest that culture temperature and pH should be precisely controlled for overproduction of KcPGA in E. coli. (c) 2005 Elsevier Inc. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | HIGH-LEVEL EXPRESSION ; GENE ; ENZYME ; INSTABILITY ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000229500800010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1857]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wen, Y,Feng, MQ,Yuan, ZY,et al. Expression and overproduction of recombinant penicillin G acylase from Kluyvera citrophila in Escherichia coli[J]. ENZYME AND MICROBIAL TECHNOLOGY,2005,37(2):233-237. |
| APA | Wen, Y,Feng, MQ,Yuan, ZY,&Zhou, P.(2005).Expression and overproduction of recombinant penicillin G acylase from Kluyvera citrophila in Escherichia coli.ENZYME AND MICROBIAL TECHNOLOGY,37(2),233-237. |
| MLA | Wen, Y,et al."Expression and overproduction of recombinant penicillin G acylase from Kluyvera citrophila in Escherichia coli".ENZYME AND MICROBIAL TECHNOLOGY 37.2(2005):233-237. |
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