Fed-batch cultivation of Escherichia coli YK537 (pAET-8) for production of phoA promoter-controlled human epidermal growth factor
文献类型：期刊论文
| 作者 | Wang, YG; Du, P; Gan, RB; Li, ZM; Ye, Q |
| 刊名 | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
 |
| 出版日期 | 2005 |
| 卷号 | 10期号:2页码:149-154 |
| 关键词 | human epidermal growth factor phoA promoter fed-batch culture Escherichia coli phosphate secretion |
| 通讯作者 | Ye, Q (reprint author), E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China.,qye@ecust.edu.cn |
| 英文摘要 | Secretion of the expressed heterologous proteins can reduce the stress to the host cells and is beneficial to their recovery and purification. in this study, fed-batch cultures of Escherichia coli YK537 (pAET-8) were conducted in a 5-L fermentor for the secretory production of human epidermal growth factor (hEGF) whose expression was under the control of alkaline phosphatase promoter. The effects of feeding of glucose and complex nitrogen sources on hEGF production were investigated. When the fed-batch culture was conducted in a chemically defined medium, the cell density was 9.68 g/L and the secreted hEGF was 44.7 mg/L in a period of 60 h. When a complex medium was used and glucose was added in pH-stat mode, the secreted hEGF was improved to 345 mg/L. When the culture was fed with glucose at a constant specific rate of 0.25 gg(-1)h(-1), hEGF reached 514 mg/L. The effects of adding a solution containing yeast extract and tryptone were further studied. Different rate of the nitrogen source feeding resulted in different levels of phosphate and acetic acid formation, thus affected hEGF expression. At the optimal feeding rate, hEGF production achieved 686 mg/L. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | OPTIMIZATION ; CULTURE ; GLUCOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228825000008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1861]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, YG,Du, P,Gan, RB,et al. Fed-batch cultivation of Escherichia coli YK537 (pAET-8) for production of phoA promoter-controlled human epidermal growth factor[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2005,10(2):149-154. |
| APA | Wang, YG,Du, P,Gan, RB,Li, ZM,&Ye, Q.(2005).Fed-batch cultivation of Escherichia coli YK537 (pAET-8) for production of phoA promoter-controlled human epidermal growth factor.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,10(2),149-154. |
| MLA | Wang, YG,et al."Fed-batch cultivation of Escherichia coli YK537 (pAET-8) for production of phoA promoter-controlled human epidermal growth factor".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 10.2(2005):149-154. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。