Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification
文献类型：期刊论文
| 作者 | Jin, WH; Dai, J; Li, SJ; Xia, QC; Zou, HF; Zeng, R |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2005 |
| 卷号 | 4期号:2页码:613-619 |
| 关键词 | proteomics human plasma mass spectrometry two-dimensional liquid chromatography protein fractionation |
| 通讯作者 | Zou, HF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Res Ctr Proteome Anal, Inst Biochem & Cell Biol,Key Lab Proteom, 320 YueYang Rd, Shanghai 200031, Peoples R China.,zouhfa@mail.dlptt.ln.cn ; zr@sibs.ac.cn |
| 英文摘要 | A resurgence of interest in the human plasma proteome has occurred in recent years because it holds great promise of revolution in disease diagnosis and therapeutic monitoring. As one of the most powerful separation techniques, multidimensional liquid chromatography has attracted extensive attention, but most published works have focused on the fractionation of tryptic peptides. In this study, proteins from human plasma were prefractionated by online sequential strong cation exchange chromatography and reversed-phase chromatography. The resulting 30 samples were individually digested by trypsin, and analyzed by capillary reversed-phase liquid chromatography coupled with linear ion trap mass spectrometry. After meeting stringent criteria, a total of 1292 distinct proteins were successfully identified in our work, among which, some proteins known to be present in serum in < 10 ng/mL were detected. Compared with other works in published literatures, this analysis offered a more full-scale list of the plasma proteome. Considering our strategy allows high throughput of protein identification in serum, the prefractionation of proteins before MS analysis is a simple and effective method to facilitate human plasma proteome research. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS ; HUMAN SERUM PROTEOME ; HEPATOCELLULAR-CARCINOMA ; CANCER-DIAGNOSIS ; OVARIAN-CANCER ; PROTEINS ; BIOMARKERS ; POPULATION ; SYSTEM ; GELS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228421900049 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1864]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jin, WH,Dai, J,Li, SJ,et al. Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification[J]. JOURNAL OF PROTEOME RESEARCH,2005,4(2):613-619. |
| APA | Jin, WH,Dai, J,Li, SJ,Xia, QC,Zou, HF,&Zeng, R.(2005).Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification.JOURNAL OF PROTEOME RESEARCH,4(2),613-619. |
| MLA | Jin, WH,et al."Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification".JOURNAL OF PROTEOME RESEARCH 4.2(2005):613-619. |
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