Structural evidence for alpha-synuclein fibrils using in situ atomic force microscopy
文献类型：期刊论文
| 作者 | Zhang, F; Ji, LN; Tang, L; Hu, J; Hui, HY; Xu, HJ; He, JH |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:2页码:113-118 |
| 关键词 | alpha-synuclein atomic force microscopy tapping mode contact mode guanidine hydrochloride |
| 通讯作者 | He, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.,hejh@sinap.ac.cn |
| 英文摘要 | Human alpha-synuclein is a presynaptic terminal protein and can form insoluble fibrils that are believed to play an important role in the pathogenesis of several neurodegenerative diseases such as Parkinson's disease, dementia with Lewy bodies and Lewy body variant of Alzheimer's disease. In this paper, in situ atomic force microscopy has been used to study the structural properties of alpha-synuclein fibrils in solution using two different atomic force microscopy imaging modes: tapping mode and contact mode. In the in situ contact mode atomic force microscopy experiments alpha-synuclein fibrils quickly broke into fragments, and a similar phenomenon was found using tapping mode atomic force microscopy in which alpha-synuclein fibrils were incubated with guanidine hydrochloride (0.6 M). The alpha-synuclein fibrils kept their original filamentous topography for over I h in the in situ tapping mode atomic force microscopy experiments. The present results provide indirect evidence on how beta-sheets assemble into alpha-synuclein fibrils on a nanometer scale. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NEURODEGENERATIVE DISORDERS ; ALZHEIMERS-DISEASE ; PROTEIN ; CONFORMATION ; AGGREGATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000226917100005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1866]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, F,Ji, LN,Tang, L,et al. Structural evidence for alpha-synuclein fibrils using in situ atomic force microscopy[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(2):113-118. |
| APA | Zhang, F.,Ji, LN.,Tang, L.,Hu, J.,Hui, HY.,...&He, JH.(2005).Structural evidence for alpha-synuclein fibrils using in situ atomic force microscopy.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(2),113-118. |
| MLA | Zhang, F,et al."Structural evidence for alpha-synuclein fibrils using in situ atomic force microscopy".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.2(2005):113-118. |
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