Identification and characterization of a novel splice variant of beta 3 subunit of GABA(A) receptor in rat testis and spermatozoa
文献类型：期刊论文
| 作者 | Li, SF; Hu, JH; Yan, YC; Chen, YG; Koide, SS; Li, YP |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:2页码:350-360 |
| 关键词 | GABA(A) receptor beta 3 subunit splice variant rat testis and spermatozoa |
| 通讯作者 | Yan, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,ycyan@sunm.shcnc.ac.cn |
| 英文摘要 | gamma-Aminobutyric acid type A (GABA(A)) receptors are the major sites of inhibitory action of fast synaptic neurotransmission in the brain. Their receptors are also widely distributed in peripheral and endocrine tissues. A full-length cDNA encoding a novel splice variant of beta3 subunit of GABA(A) receptor, designated as beta3t, was identified in rat testis. This isoform contains a segment, having identical amino acid sequence as the beta3 subunit of neuronal GABA(A) receptors except for a section composed of 25 different amino acid sequence in the N-terminus. Northern blot shows that this isoform is found in rat testis. The beta3t isoform mRNA was detected in germ cells in the late step of spermatogenesis by in situ hybridization assay. Results of immunohistochemical and immunocytochemical assays indicate that the beta3t isoform is expressed in rat testis and spermatozoa. To determine a possible function of the N-terminal 25 amino acid segment, a recombinant plasmid of beta3t-EGFPC was constructed by fusing green fluorescent protein to the C-terminus of the beta3t isoform. The chimera product failed to be translocated unto the cell surface when expressed in HEK 293 cells; whereas, the beta3 subunit of rat brain is incorporated into the plasma membrane. In conclusion, the present results show that one variant of beta3 subunit of GABA(A) receptor, designated as beta3t, is found in germ cells of rat testis and sperm. The inability of the beta3t variant to target into the plasma membrane maybe a consequence of the unique 25 amino acid segment in the N-terminus. (C) 2004 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | MESSENGER-RNA ; EXPRESSION ; SYSTEM ; SPERM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224909400013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1869]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, SF,Hu, JH,Yan, YC,et al. Identification and characterization of a novel splice variant of beta 3 subunit of GABA(A) receptor in rat testis and spermatozoa[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2005,37(2):350-360. |
| APA | Li, SF,Hu, JH,Yan, YC,Chen, YG,Koide, SS,&Li, YP.(2005).Identification and characterization of a novel splice variant of beta 3 subunit of GABA(A) receptor in rat testis and spermatozoa.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,37(2),350-360. |
| MLA | Li, SF,et al."Identification and characterization of a novel splice variant of beta 3 subunit of GABA(A) receptor in rat testis and spermatozoa".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 37.2(2005):350-360. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。