P53 protein activates the transcription of human proliferating cell nuclear antigen in response to 4-nitroquinoline N-oxide treatment
文献类型：期刊论文
| 作者 | Li, YY; Wang, L; Li, SH; Guo, TQ; Guo, XY; Yan, PJ; Chen, Y; Wang, LZ; Lu, CD |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:2页码:416-426 |
| 关键词 | 4-nitroquinoline N-oxide proliferating cell nuclear antigen p53 DNA damage cell cycle checkpoint |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,cdlu@sibs.ac.cn |
| 英文摘要 | 4-NitroquinolineN-oxide(4-NQO) is a potent mutagen and carcinogen. To elucidate the cellular response to 4-NQO, we studied the transcriptional regulation of human proliferating cell nuclear antigen (hPCNA), an essential protein in DNA replication and repair, after 4-NQO treatment. We found that hPCNA promoter was dose-dependently transactivated by 4-NQO under the concentration of 2 muM via a previously reported p53-binding element located from -236 to -217 upstream of the transcription start site. Based on our western blot analysis, the phosphorylation of serine at the 15th residue (Ser15) of p53 was activated by 4-NQO, whereas the level of p53 in the cells did not change much. It was observed that Staurosporine, a Ser/Thr kinase inhibitor, blocked the Ser15 phosphorylation of p53 and the hPCNA promoter response to 4-NQO simultaneously, suggesting that Ser15 phosphorylated p53 was the 4-NQO-responsive hPCNA regulator. The [H-3]-thymidine deoxyribose (TdR) incorporation assay and the comet assay showed that DNA repair was triggered when DNA replication was inhibited after the treatment of 4-NQO, and the hPCNA transactivation seemed to contribute to DNA repair. Taken together, our data indicate that after 4-NQO treatment hPCNA is transactivated by Ser15 phosphorylated p53, and participate in DNA repair. (C) 2004 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | DAMAGE-INDUCED PHOSPHORYLATION ; DNA-DAMAGE ; EXCISION-REPAIR ; PCNA PROMOTER ; INHIBITION ; MDM2 ; GENE ; REPLICATION ; DEGRADATION ; ONCOPROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224909400019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1870]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, YY,Wang, L,Li, SH,et al. P53 protein activates the transcription of human proliferating cell nuclear antigen in response to 4-nitroquinoline N-oxide treatment[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2005,37(2):416-426. |
| APA | Li, YY.,Wang, L.,Li, SH.,Guo, TQ.,Guo, XY.,...&Lu, CD.(2005).P53 protein activates the transcription of human proliferating cell nuclear antigen in response to 4-nitroquinoline N-oxide treatment.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,37(2),416-426. |
| MLA | Li, YY,et al."P53 protein activates the transcription of human proliferating cell nuclear antigen in response to 4-nitroquinoline N-oxide treatment".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 37.2(2005):416-426. |
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