Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing
文献类型：期刊论文
| 作者 | Cheng, GF; Lin, JJ; Feng, XG; Fu, ZQ; Jin, YM; Yuan, CX; Zhou, YC; Cai, YM |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2005 |
| 卷号 | 5期号:2页码:511-521 |
| 关键词 | differentially expressed proteins Schistosoma japonicum proteome pairing two-dimensional gel electrophoresis |
| 通讯作者 | Cai, YM (reprint author), Chinese Acad Agr Sci, Shanghai Inst Anim Parasitol, Key Lab Anim Parasitol, Minist Agr, 3,Lane 345,Shilong Rd, Shanghai 200232, Peoples R China.,caassp@public.sta.net.cn |
| 英文摘要 | Identification of differentially expressed proteins between the male and female worm of Schistosoma japonicum may provide new insights into the development of schistosomes, especially the molecular mechanism of female worm maturation induced by the male worm after pairing. Comparative two-dimensional gel electrophoresis (2-DE) and mass spectrometry were employed to separate and identify differentially expressed proteins between the male and female worm after pairing. Soluble and hydrophobic proteins from egg, schistosomulum (14 days), and female and male worms at adult stage (42 days) were separated by a sequential extraction method followed by 2-DE and 2-DE images were constructed. There were 1016 +/- 67, 1808 +/- 89, 1142 +/- 45 and 1288 +/- 32 spots detected for soluble proteins and 1425 +/- 108, 952 +/- 59, 847 +/- 75 and 965 +/- 69 spots for hydrophobic proteins from egg, schistosomulum, and adult stage female and male worms, respectively. The differentially and uniquely expressed proteins from male and female worms after pairing (42 days) include 41 +/- 4 and 23 +/- 2 unique spots for soluble proteins, and 11 +/- 1 and 26 +/- 3 unique spots for hydrophobic proteins, respectively. Matrix-assisted laser desorption/ionization-time of flight and electrospray ionization-tandem mass spectrometry were employed to analyze 12 unique spots from the female worm and 16 unique spots from the male worm for peptide mass fingerprinting and sequencing. The results showed that the main functions of these differentially expressed proteins were in signal transduction, metabolism and transcriptional regulation etc. Comparison of the schistosomes proteome between male and female worms may permit the identification of protein candidates for the development of vaccines or new targets for drug development against schistosomiasis. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 关键词[WOS] | GENE-EXPRESSION ; MANSONI ; DNA ; STIMULATION ; GRADIENTS ; IMMATURE ; INVITRO ; GENOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227112100020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1872]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, GF,Lin, JJ,Feng, XG,et al. Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing[J]. PROTEOMICS,2005,5(2):511-521. |
| APA | Cheng, GF.,Lin, JJ.,Feng, XG.,Fu, ZQ.,Jin, YM.,...&Cai, YM.(2005).Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing.PROTEOMICS,5(2),511-521. |
| MLA | Cheng, GF,et al."Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosomal japonicum after pairing".PROTEOMICS 5.2(2005):511-521. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。