High level expression of functionally active human lactoferrin in silkworm larvae
文献类型：期刊论文
| 作者 | Liu, T; Zhang, YZ; Wu, XF |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 118期号:3页码:246-256 |
| 关键词 | human lactoferrin silkworm larvae recombinant baculovirus colitis |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Sci Tech Univ, Inst Biochem, Hangzhou 310018, Peoples R China.,liuniu73@yahoo.com.cn |
| 英文摘要 | In this paper, recombinant human lactoferrin (rhLf) was expressed very well using Bombyx mori nuclear polyhedrosis baculovirus expression system. Infection of silkworm larvae with recombinant virus, vBm-hLf, the rhLf was efficiently secreted into larvae hemolymph and the concentration of product purified was about 65 mu g/ml. The isolated rhLf molecular mass was similar to 78 kDa, lower than that of the human lactoferrin (hLf) standards, which may be due to incomplete glycosylation or protein degradation. Furthermore, the rhLf was characterized and its biological activities were evaluated by in vivo bioassay using dextran sodium sulfate (DSS)-induced colitis mouse model that mimics some characteristics of colitis disease in human. We conclude that silkworm expression system can be used successfully to express functional human lactoferrin. (C) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | INFLAMMATORY-BOWEL-DISEASE ; BACULOVIRUS VECTOR ; COLITIS ; MICE ; RECEPTORS ; CELLS ; MILK |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231124200002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1875]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, T,Zhang, YZ,Wu, XF. High level expression of functionally active human lactoferrin in silkworm larvae[J]. JOURNAL OF BIOTECHNOLOGY,2005,118(3):246-256. |
| APA | Liu, T,Zhang, YZ,&Wu, XF.(2005).High level expression of functionally active human lactoferrin in silkworm larvae.JOURNAL OF BIOTECHNOLOGY,118(3),246-256. |
| MLA | Liu, T,et al."High level expression of functionally active human lactoferrin in silkworm larvae".JOURNAL OF BIOTECHNOLOGY 118.3(2005):246-256. |
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