Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2
文献类型：期刊论文
| 作者 | Liang, AY; He, XY; Du, YG; Wang, KY; Fung, YS; Lin, BC |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2005 |
| 卷号 | 38期号:3页码:408-413 |
| 关键词 | low molecular weight heparin interleukin 2 capillary zone electrophoresis interaction |
| 通讯作者 | Lin, BC (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China.,bclin@dicp.ac.cn |
| 英文摘要 | A method based on capillary zone electrophoresis (CZE) was used to study the interaction between low molecular weight heparin (LMWH) and interleukin 2 (IL-2). The results showed that the increase of the concentration of LMWH led to the decrease of the peak height and the increase of the peak width of IL-2, but the peak areas were kept constant. The binding constant of IL-2 with LMWH was calculated as 1.2 x 10(6) M(-1) by Scatchard analysis, which is in good agreement with the results found in the references using enzyme-linked immunosorbent assay (ELISA). The results demonstrated that the interaction between IL-2 and LMWH is of fast on-and-off kinetic binding reaction. CZE might be used to study not only slow on-and-off rates interactions, but also fast on-and-off rates ones. The binding constant can be calculated easily, and the method can be applied to study a wide range of heparin-protein interactions. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Analytical ; Pharmacology & Pharmacy |
| 关键词[WOS] | FIBROBLAST-GROWTH-FACTOR ; HUMAN RECOMBINANT INTERLEUKIN-2 ; AMYLOID-P COMPONENT ; ASSOCIATION CONSTANTS ; ANTITHROMBIN-BINDING ; COMPLEX-FORMATION ; PROTEIN-BINDING ; AFFINITY ; DOMAIN ; DRUG |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230235200004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1878]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liang, AY,He, XY,Du, YG,et al. Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2005,38(3):408-413. |
| APA | Liang, AY,He, XY,Du, YG,Wang, KY,Fung, YS,&Lin, BC.(2005).Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,38(3),408-413. |
| MLA | Liang, AY,et al."Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 38.3(2005):408-413. |
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