Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs
文献类型：期刊论文
| 作者 | Shi, Y; Yang, DH; Xiong, J; Jia, H; Huang, B; Jin, YX |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2005 |
| 卷号 | 15期号:3页码:193-200 |
| 关键词 | SARS small interfering RNA Vero E6 cells EGFP fusion protein antiviral therapy |
| 通讯作者 | Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,yxjin@sibs.ac.cn |
| 英文摘要 | RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3' overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0 similar to 60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5' end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; VIRUS TYPE-1 REPLICATION ; DOUBLE-STRANDED-RNA ; HEPATITIS-B-VIRUS ; GENOME SEQUENCE ; CELLS ; INFECTION ; PLANTS ; PROTEIN ; SIRNAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228260800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1881]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Shi, Y,Yang, DH,Xiong, J,et al. Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs[J]. CELL RESEARCH,2005,15(3):193-200. |
| APA | Shi, Y,Yang, DH,Xiong, J,Jia, H,Huang, B,&Jin, YX.(2005).Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs.CELL RESEARCH,15(3),193-200. |
| MLA | Shi, Y,et al."Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs".CELL RESEARCH 15.3(2005):193-200. |
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