Effect of RNA interference on Gal alpha 1,3 Gal expression in PIEC cells
文献类型：期刊论文
| 作者 | Yu, LY; Miao, HS; Guo, LH |
| 刊名 | DNA AND CELL BIOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 24期号:3页码:180-188 |
| 通讯作者 | Yu, LY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 20031, Peoples R China.,lhguo@sibs.ac.cn |
| 英文摘要 | Xenotransplantation from pig to human being is viewed as a potential solution for the acute organ shortage. However, consequent xenorejection induced by Gal alpha 1,3 Gal ( Gal, Gal antigen) prevents xenotransplantation from clinical application. Thus, the most attracting attempt to prevent xenorejection is the elimination of Gal. Our study suggested that compared with the human alpha 1,2 fucosyltransferase ( FT) gene and porcine antisense alpha 1,3 galactosyltransferase gene, sequence-specific siRNA targeting Gal were capable of suppressing Gal expression markedly, and therefore, significantly inhibiting xenoreactivity and the complement activation with human serum in PIEC cells. We also demonstrated the concordant inhibitory effect of siRNA and human FT gene on Gal and corresponding functions, which implied a practical significance of combined transgenic strategy. The successful application of vector-based dsRNA-GT may extend the list of available modalities in the abrogation of xenorejection in xenotransplantation. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology; Genetics & Heredity |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology ; Genetics & Heredity |
| 关键词[WOS] | ACUTE VASCULAR REJECTION ; ALPHA-GALACTOSIDASE ; HUMAN SERUM ; MEDIATED CYTOLYSIS ; PIG-CELLS ; ANTIBODIES ; ALPHA-1,3-GALACTOSYLTRANSFERASE ; GENE ; SURFACE ; ALPHA-1,2-FUCOSYL-TRANSFERASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227626000005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1882]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, LY,Miao, HS,Guo, LH. Effect of RNA interference on Gal alpha 1,3 Gal expression in PIEC cells[J]. DNA AND CELL BIOLOGY,2005,24(3):180-188. |
| APA | Yu, LY,Miao, HS,&Guo, LH.(2005).Effect of RNA interference on Gal alpha 1,3 Gal expression in PIEC cells.DNA AND CELL BIOLOGY,24(3),180-188. |
| MLA | Yu, LY,et al."Effect of RNA interference on Gal alpha 1,3 Gal expression in PIEC cells".DNA AND CELL BIOLOGY 24.3(2005):180-188. |
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