Synergistic activity of sef and sprouty proteins in regulating the expression of Gbx2 in the mid-hindbrain region
文献类型：期刊论文
| 作者 | Lin, W; Jing, NH; Basson, MA; Dierich, A; Licht, J; Ang, SL |
| 刊名 | GENESIS
 |
| 出版日期 | 2005 |
| 卷号 | 41期号:3页码:110-115 |
| 关键词 | sprouty2 dominant negative Sef electroporation embryo culture mid-hindbrain development |
| 通讯作者 | Ang, SL (reprint author), Natl Inst Med Res, MRC, Div Dev Neurobiol, Ridgeway,Mill Hill, London NW7 1AA, England.,sang@nimr.mrc.ac.uk |
| 英文摘要 | Sef and Sprouty proteins function as feedback antagonists of fibroblast growth factor (Fgf) signaling in zebrafish embryos. To study the role of Sef in mice, we generated Sef homozygous mutant animals. These animals are viable and show normal expression of mid-hindbrain genes at embryonic days 8.5 and 9.5. To investigate the possibility of functional synergism between Sef and Sprouty proteins, we electroporated Sprouty2(Y55A), which functions in a dominant-negative manner in tissue culture cells into the mid-hindbrain region of wildtype and Sef mutant embryos. The expression pattern of Gbx2, a downstream target of Fgf signaling, was expanded or shifted in electroporated embryos, and this effect was significantly enhanced in the Sef mutant background. Altogether, our results demonstrate that Sef and Sproutys function synergistically to regulate Gbx2 expression in the anterior hindbrain. genesis 41:110-115, 2005. (c) 2005 Wiley-Liss, Inc. |
| 学科主题 | Developmental Biology; Genetics & Heredity |
| 类目[WOS] | Developmental Biology ; Genetics & Heredity |
| 关键词[WOS] | ISTHMIC ORGANIZER ; CELL-SURVIVAL ; FGF8 ; GENE ; CEREBELLUM ; ANTAGONIST ; MIDBRAIN ; ELECTROPORATION ; IDENTIFICATION ; ENCODES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228176700002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1883]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Lin, W,Jing, NH,Basson, MA,et al. Synergistic activity of sef and sprouty proteins in regulating the expression of Gbx2 in the mid-hindbrain region[J]. GENESIS,2005,41(3):110-115. |
| APA | Lin, W,Jing, NH,Basson, MA,Dierich, A,Licht, J,&Ang, SL.(2005).Synergistic activity of sef and sprouty proteins in regulating the expression of Gbx2 in the mid-hindbrain region.GENESIS,41(3),110-115. |
| MLA | Lin, W,et al."Synergistic activity of sef and sprouty proteins in regulating the expression of Gbx2 in the mid-hindbrain region".GENESIS 41.3(2005):110-115. |
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