Macro-branched cell-penetrating peptide design for gene delivery
文献类型：期刊论文
| 作者 | Liu, ZH; Li, MY; Cui, DF; Fei, J |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2005 |
| 卷号 | 102期号:3页码:699-710 |
| 关键词 | macro-branched peptide HIV-1 tat gene delivery dendrimer non-viral vector |
| 通讯作者 | Fei, J (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,jfei@sibs.ac.cn |
| 英文摘要 | Many kinds of macromolecular compounds that comprise a series of branches around an inner core have been made and showed some promises as DNA- and drug-delivery systems. In this study, a method for peptide polymer design having macro-branched peptide structure was adapted and it was applied for plasmid DNA delivery for the first time; acryloyl chloride was used by introducing the double bond into the N-terminal of HIV-1 tat (47-57, YGRKKRRQRRR). After being cleaved from resin, it was transformed to a polymer which possesses a poly-propionyl core matrix with tat branches and was named polytat by radical polymerization. After further separation and purification by Sephadex G150, polytat P1, polytat P2 and polytat P3 were acquired and the average molecular weight of the different macromolecules are 240, 178 and 82 kDa. We found that all these compounds complexed with plasmid DNA and showed significant transfection capabilities in a variety of mammalian cell lines, but acrylyl-tat or tat alone showed no significant transfection capability. Further study showed that polytat could be applied for plasmid DNA delivery for cell lines even with serum by endocytosis-mediated pathways. (C) 2004 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | HIV-1 TAT PROTEIN ; IN-VIVO ; TRANSFECTION EFFICIENCY ; MOLECULAR-WEIGHT ; DNA COMPLEXES ; VECTOR ; POLYETHYLENIMINE ; NANOPARTICLES ; CYTOTOXICITY ; TRANSDUCTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227146500016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1886]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, ZH,Li, MY,Cui, DF,et al. Macro-branched cell-penetrating peptide design for gene delivery[J]. JOURNAL OF CONTROLLED RELEASE,2005,102(3):699-710. |
| APA | Liu, ZH,Li, MY,Cui, DF,&Fei, J.(2005).Macro-branched cell-penetrating peptide design for gene delivery.JOURNAL OF CONTROLLED RELEASE,102(3),699-710. |
| MLA | Liu, ZH,et al."Macro-branched cell-penetrating peptide design for gene delivery".JOURNAL OF CONTROLLED RELEASE 102.3(2005):699-710. |
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