Prefractionation of proteome by liquid isoelectric focusing prior to two-dimensional liquid chromatography mass spectrometric identification
文献类型：期刊论文
| 作者 | Li, RX; Zhou, H; Li, SJ; Sheng, QH; Xia, QC; Zeng, R |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2005 |
| 卷号 | 4期号:4页码:1256-1264 |
| 关键词 | large-scale proteome fractionation liquid isoelectric focusing (LIEF) 2D-LC-MS/MS low-abundance proteins |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom,Res Ctr Proteome Anal, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn |
| 英文摘要 | Due to the complexity of proteomes, developing methods of sample fractionation, separation, concentration, and detection have become urgent to the identification of large numbers of proteins, as well as the acquisition of those proteins in low abundance. In this work, liquid isoelectric focusing (LIEF) combined with 2D-LC-MS/MS was applied to the proteome of Saccharomyces cerevisiae. This yielded a total of 1795 proteins that were detected and identified by 30 fractions of protein prefractioantion. Categorization of these hits demonstrated the ability of this technology to detect and identify proteins rarely seen in proteome analysis without protein fractiontion. LIEF-2D-LC-IVIS/lVIS also produced improved resolution of low-abundance proteins. Furthermore, we analyzed the characteristics of proteins obtained by LIEF-2D-LC-MVS/MVS. 1103 proteins with CAI under 0.2 were identified, allowing us to specifically obtain detailed biochemical information on these kind proteins. It was observed that LIEF-2D-LC-MVS/MVS is useful for large-scale proteome analysis and may be specifically applied to systems with wide dynamic ranges. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | POLYACRYLAMIDE-GEL ELECTROPHORESIS ; SOLUTION ISOELECTROFOCUSING PRIOR ; LASER-DESORPTION/IONIZATION-TIME ; NATURAL PH GRADIENTS ; SHOTGUN PROTEOMICS ; HIGH-RESOLUTION ; SAMPLE PREFRACTIONATION ; IMMOBILINE MEMBRANES ; SEPARATION METHOD ; YEAST PROTEOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231184200023 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1890]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, RX,Zhou, H,Li, SJ,et al. Prefractionation of proteome by liquid isoelectric focusing prior to two-dimensional liquid chromatography mass spectrometric identification[J]. JOURNAL OF PROTEOME RESEARCH,2005,4(4):1256-1264. |
| APA | Li, RX,Zhou, H,Li, SJ,Sheng, QH,Xia, QC,&Zeng, R.(2005).Prefractionation of proteome by liquid isoelectric focusing prior to two-dimensional liquid chromatography mass spectrometric identification.JOURNAL OF PROTEOME RESEARCH,4(4),1256-1264. |
| MLA | Li, RX,et al."Prefractionation of proteome by liquid isoelectric focusing prior to two-dimensional liquid chromatography mass spectrometric identification".JOURNAL OF PROTEOME RESEARCH 4.4(2005):1256-1264. |
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