Suppression of tumor growth by oncolytic adenovirus-mediated delivery of an antiangiogenic gene, soluble Flt-1
文献类型：期刊论文
| 作者 | Zhang, ZL; Zou, WG; Wang, JH; Gu, JF; Dang, YK; Li, BH; Zhao, LL; Qian, C; Qian, QJ; Liu, XY |
| 刊名 | MOLECULAR THERAPY
 |
| 出版日期 | 2005 |
| 卷号 | 11期号:4页码:553-562 |
| 关键词 | oncolytic adenovirus E1B-55-kDa gene antiangiogenesis sflt-1(1-3) gene gene-viral therapy |
| 通讯作者 | Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn |
| 英文摘要 | Armed oncolytic adenoviruses represent an appealing tumor treatment approach, as they can attack tumors at multiple levels. In this study, considering that angiogenesis plays a central role in tumor growth, we inserted an antiangiogenic gene, sflt-7(1-3) (the first three extracellular domains of FLT1, the hVEGF receptor-1), into an E1B-55-kDa-deleted oncolytic adenovirus (ZD55) to construct ZD55-sflt-1. Although soluble (s) Flt-1 did not affect tumor cell growth, ZD55-sflt-1 could specifically induce a cytopathic effect in tumor cells, like ONYX-015. The secretion of sFlt-1 from ZD55-sflt-1 was much higher than that from replication-deficient Ad-sflt-1 upon infection of SW620 human colon tumor cells, leading to a stronger inhibitory effect on VEGF-induced proliferation and tube formation ability of HUVECs. Moreover, marked reduction of tumor growth and long-term survival rates were observed in ZD55-sflt-1-treated nude mice with subcutaneous SW620 tumor. Its efficacy correlated with a decrease in microvessel density and an increase in apoptotic tumor cells. In addition, ZD55-sflt-1 showed a synergic effect with the chemotherapeutic agent 5-FU. These results indicate that ZD55-sflt-1, combining the advantages of oncolytic adenovirus and antiangiogenic gene therapy, is a powerful agent for human tumor treatment. |
| 学科主题 | Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 关键词[WOS] | FACTOR RECEPTOR ; REPLICATING ADENOVIRUS ; COMPETENT ADENOVIRUS ; EXTRACELLULAR DOMAIN ; ANTITUMOR EFFICACY ; CANCER-THERAPY ; P53 STATUS ; IN-VIVO ; ANGIOGENESIS ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227991700010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1900]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, ZL,Zou, WG,Wang, JH,et al. Suppression of tumor growth by oncolytic adenovirus-mediated delivery of an antiangiogenic gene, soluble Flt-1[J]. MOLECULAR THERAPY,2005,11(4):553-562. |
| APA | Zhang, ZL.,Zou, WG.,Wang, JH.,Gu, JF.,Dang, YK.,...&Liu, XY.(2005).Suppression of tumor growth by oncolytic adenovirus-mediated delivery of an antiangiogenic gene, soluble Flt-1.MOLECULAR THERAPY,11(4),553-562. |
| MLA | Zhang, ZL,et al."Suppression of tumor growth by oncolytic adenovirus-mediated delivery of an antiangiogenic gene, soluble Flt-1".MOLECULAR THERAPY 11.4(2005):553-562. |
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