Expression and transport function of the glutamate transporter EAAC1 in Xenopus oocytes is regulated by syntaxin 1A
文献类型：期刊论文
| 作者 | Zhu, Y; Fei, J; Schwarz, W |
| 刊名 | JOURNAL OF NEUROSCIENCE RESEARCH
 |
| 出版日期 | 2005 |
| 卷号 | 79期号:4页码:503-508 |
| 关键词 | neurotransmitter transporter Xenopus oocyte glutamate uptake EAAC1-mediated current |
| 通讯作者 | Schwarz, W (reprint author), Max Planck Inst Biophys, Marie Curie Str 15, D-60439 Frankfurt, Germany.,schwarz@mpibp-frankfurt.mpg.de |
| 英文摘要 | The function of several membrane proteins is regulated by interaction with the SNARE protein syntaxin 1A; this includes regulation of GAT1, the transporter for the dominating inhibitory neurotransmitter gamma-aminobutyric acid (GABA). Here we demonstrate that also EAAC1, the transporter for the dominating excitatory neurotransmitter, is down-regulated by interaction with syntaxin 1A. This is shown by coexpression of EAAC1 and syntaxin 1A in Xenopus oocytes. Total EAAC1 expression is not significantly affected by the coexpression of syntaxin 1A, but more proteins become targeted to the membrane as demonstrated by biotinylation. Colocalization by coimmunoprecipitation suggests direct interaction between the two proteins. In contrast to the number of transporters, the glutamate transport activity becomes reduced, and even stronger inhibition is observed for the EAAC1 mediated conductance uncoupled from glutamate translocation. We conclude that the interaction of syntaxin 1A with EAAC1 particularly disrupts the structure of the conductance pathway of EAAC1. (C) 2004 Wiley-Liss, Inc. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | EPITHELIAL SODIUM-CHANNEL ; AMINO-ACID TRANSPORTER ; PROTEIN-KINASE-C ; CALCIUM CHANNELS ; GABA TRANSPORTER ; RAT-BRAIN ; N-TYPE ; 1A ; RECEPTORS ; FUSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000226677200010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1906]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, Y,Fei, J,Schwarz, W. Expression and transport function of the glutamate transporter EAAC1 in Xenopus oocytes is regulated by syntaxin 1A[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2005,79(4):503-508. |
| APA | Zhu, Y,Fei, J,&Schwarz, W.(2005).Expression and transport function of the glutamate transporter EAAC1 in Xenopus oocytes is regulated by syntaxin 1A.JOURNAL OF NEUROSCIENCE RESEARCH,79(4),503-508. |
| MLA | Zhu, Y,et al."Expression and transport function of the glutamate transporter EAAC1 in Xenopus oocytes is regulated by syntaxin 1A".JOURNAL OF NEUROSCIENCE RESEARCH 79.4(2005):503-508. |
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