Purification and application of C-terminally truncated hepatitis C virus E1 proteins expressed in Escherichia coli
文献类型：期刊论文
| 作者 | Liu, J; Zhu, LX; Kong, YY; Li, GD; Wang, Y |
| 刊名 | WORLD JOURNAL OF GASTROENTEROLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 11期号:4页码:503-507 |
| 关键词 | HCV envelope protein 1 Recombinant Fusion Proteins Escherichia coli |
| 通讯作者 | Wang, Y (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,wangyuan@shcnc.ac.cn |
| 英文摘要 | AIM: To explore the possibility of expressing hepatitis C virus (HCV) envelope protein 1 (E1) in Escherichia coli (E. coli) and to test the purified recombinant E1 proteins for clinical and research applications. METHODS: C-terminally truncated E1 fragments were expressed in E. coli as hexa-histidine-tagged fusion proteins. The expression products were purified under denaturing conditions using immobilized-metal affinity chromatography. Purified E1 proteins were used to immunize rabbits. Rabbit anti-sera thus obtained were reacted with both E. coli-and mammalian cell-expressed E1 glycoproteins as detected by Western blot. RESULTS: Full-length E1 protein proved difficult to express in E.coli. C-terminally truncated E1 was successfully expressed in E. coli as hexa-histidine-tagged recombinant fusion protein and was purified under denaturing conditions on Ni(2+)-NTA agarose. Rabbit anti-sera raised against purified recombinant E1 specifically reacted with mammalian cell-expressed E1 glycoproteins in Western blot. Furthermore, E.coli-derived E1 protein was able to detect animal antibodies elicited by E1-based DNA immunization. CONCLUSION: These results demonstrate that the prokaryotically expressed E1 proteins share identical epitopes with eukaryotically expressed E1 glycoprotein. The E.coli-derived E1 proteins and corresponding antisera can become useful tools in anti-HCV vaccine research. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved. |
| 学科主题 | Gastroenterology & Hepatology |
| 类目[WOS] | Gastroenterology & Hepatology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000208099700008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1907]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, J,Zhu, LX,Kong, YY,et al. Purification and application of C-terminally truncated hepatitis C virus E1 proteins expressed in Escherichia coli[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(4):503-507. |
| APA | Liu, J,Zhu, LX,Kong, YY,Li, GD,&Wang, Y.(2005).Purification and application of C-terminally truncated hepatitis C virus E1 proteins expressed in Escherichia coli.WORLD JOURNAL OF GASTROENTEROLOGY,11(4),503-507. |
| MLA | Liu, J,et al."Purification and application of C-terminally truncated hepatitis C virus E1 proteins expressed in Escherichia coli".WORLD JOURNAL OF GASTROENTEROLOGY 11.4(2005):503-507. |
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