Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis
文献类型：期刊论文
| 作者 | Cai, XH; Zhang, Q; Shi, SY; Ding, DF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:5页码:293-302 |
| 关键词 | two-component system phosphorelay histidine kinase response regulator three-dimensional cluster analysis |
| 通讯作者 | Ding, DF (reprint author), Chinese Acad Sci, Key Lab Proteom, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,Grad Sch, Shanghai 200031, Peoples R China.,dingdafu@server.shcnc.ac.cn |
| 英文摘要 | Two-component and phosphorelay signal transduction systems are central components in the virulence and antimicrobial resistance responses of a number of bacterial and fungal pathogens; in some cases, these systems are essential for bacterial growth and viability. Herein, we analyze in detail the conserved surface residue clusters in the phosphotransferase domain of histidine kinases and the regulatory domain of response regulators by using complex structure-based three-dimensional cluster analysis. We also investigate the protein-protein interactions that these residue clusters participate in. The Spo0B-Spo0F complex structure was used as the reference structure, and the multiple aligned sequences of phosphotransferases and response regulators were paired correspondingly. The results show that a contiguous conserved residue cluster is formed around the active site, which crosses the interface of histidine kinases and response regulators. The conserved residue clusters of phosphotransferase and the regulatory domains are directly involved in the functional implementation of two-component signal transduction systems and are good targets for the development of novel antimicrobial agents. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RESPONSE REGULATOR PHOSPHOTRANSFERASE ; MOLECULAR RECOGNITION ; ESCHERICHIA-COLI ; GLYCOPEPTIDE RESISTANCE ; BACTERIAL CHEMOTAXIS ; PROTEIN CHEY ; ACTIVE-SITE ; PHOSPHORYLATION ; PHOSPHATASE ; MECHANISM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000229304400002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1914]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cai, XH,Zhang, Q,Shi, SY,et al. Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(5):293-302. |
| APA | Cai, XH,Zhang, Q,Shi, SY,&Ding, DF.(2005).Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(5),293-302. |
| MLA | Cai, XH,et al."Searching for potential drug targets in two-component and phosphorelay signal-transduction systems using three-dimensional cluster analysis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.5(2005):293-302. |
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