Isolation and characterization of the murine Nanog gene promoter
文献类型：期刊论文
| 作者 | Wu, DY; Yao, Z |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2005 |
| 卷号 | 15期号:5页码:317-324 |
| 关键词 | Nanog promoter F9 EC cells Oct-1 Oct-4 Oct-6 |
| 通讯作者 | Wu, DY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Stem Cell Biol,Lab Mol Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,dywu@sibs.ac.cn |
| 英文摘要 | Nanog protein is expressed in the interior cells of compacted morulae and maintained till epiblasts but downregulated by implantation stage. It is also expressed in embryonic stem cells, embryonic carcinoma cells and embryonic germ cells but disappeared in differentiated ES cells. In this study, we have isolated, sequenced, and performed the first characterization of the Nanog promoter. The transcription start sites were mapped by primer extension analysis. Two promoter regions were found upstream the transcription start sites and the expression of major Nanog promoter/reporter gene construct is abolished in differentiated F9 EC cells as compared to the undifferentiated counterpart. We also showed that a putative octamer motif (ATGCAAAA) is necessary for the major promoter activity. Gel shift and supershift assays showed that Oct-1, Oct-4 and Oct-6 protein selectively bind to the octamer motif. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; ES CELLS ; TRANSCRIPTION ; EXPRESSION ; DIFFERENTIATION ; PLURIPOTENCY ; OCT-4 ; PROTEINS ; ELEMENT ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230838800002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1917]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wu, DY,Yao, Z. Isolation and characterization of the murine Nanog gene promoter[J]. CELL RESEARCH,2005,15(5):317-324. |
| APA | Wu, DY,&Yao, Z.(2005).Isolation and characterization of the murine Nanog gene promoter.CELL RESEARCH,15(5),317-324. |
| MLA | Wu, DY,et al."Isolation and characterization of the murine Nanog gene promoter".CELL RESEARCH 15.5(2005):317-324. |
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