Pedtidomics of Cpe(fat/fat) mouse hypothalamus - Effect of food deprivation and exercise on peptide levels
文献类型：期刊论文
| 作者 | Che, FY; Yuan, Q; Kalinina, E; Fricker, LD |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 280期号:6页码:4451-4461 |
| 通讯作者 | Fricker, LD (reprint author), Albert Einstein Coll Med, Dept Mol Pharmacol, 1300 Morrris Pk Ave, Bronx, NY 10461 USA.,fricker@aecom.yu.edu |
| 英文摘要 | Carboxypeptidase E is a major enzyme in the biosynthesis of numerous neuroendocrine peptides. Previously, we developed a technique for the isolation of neuropeptide-processing intermediates from mice that lack carboxypeptidase E activity Cpe(fat/fat) mice) due to a naturally occurring point mutation. In the present study, we used a differential labeling procedure with stable isotopic tags and mass spectrometry to quantitate the relative changes in a number of hypothalamic peptides in Cpe(fat/fat) mice in two different paradigms that each cause an similar to10% decrease in body mass. One paradigm involved a 2-day fast under normal sedentary conditions (i.e. standard mouse cages); the other involved giving mice access to an exercise wheel for 4 weeks with free access to food. Approximately 50 peptides were detected in both studies, and over 80 peptides were detected in at least one of the two studies. Twenty-eight peptides were increased >50% by food deprivation, and some of these were increased by 2- to 3-fold. In contrast, only three peptides were increased >50% in the group with exercise wheels, and many peptides showed a slight 15-30% decrease upon exercise. Approximately one-half of the peptides detected in both studies were identified by tandem mass spectrometry. Peptides found to be elevated by food deprivation but not exercise included a number of fragments of proenkephalin, prothyrotropin-releasing hormone, secretogranin II, chromogranin B, and pro-SAAS. Taken together, the differential regulation of these peptides in the two paradigms suggests that the regulation is not due to the lower body weight but to the manner in which the paradigms achieved this lower body weight. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MELANIN-CONCENTRATING HORMONE ; OBESE FAT/FAT MICE ; CARBOXYPEPTIDASE-E ; MASS-SPECTROMETRY ; METALLOCARBOXYPEPTIDASE D ; CPE(FAT)/CPE(FAT) MICE ; PROPROTEIN CONVERTASES ; MESSENGER-RNA ; ATT-20 CELLS ; PROSAAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227096600056 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1930]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Che, FY,Yuan, Q,Kalinina, E,et al. Pedtidomics of Cpe(fat/fat) mouse hypothalamus - Effect of food deprivation and exercise on peptide levels[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2005,280(6):4451-4461. |
| APA | Che, FY,Yuan, Q,Kalinina, E,&Fricker, LD.(2005).Pedtidomics of Cpe(fat/fat) mouse hypothalamus - Effect of food deprivation and exercise on peptide levels.JOURNAL OF BIOLOGICAL CHEMISTRY,280(6),4451-4461. |
| MLA | Che, FY,et al."Pedtidomics of Cpe(fat/fat) mouse hypothalamus - Effect of food deprivation and exercise on peptide levels".JOURNAL OF BIOLOGICAL CHEMISTRY 280.6(2005):4451-4461. |
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