Preparation and preliminary application of monoclonal antibodies against Trichokirin-S1, a small ribosome-inactivating peptide from the seeds of Trichosanthes kirilowii
文献类型：期刊论文
| 作者 | Yang, XX; Li, F; Hu, WG; Xia, HC; Zhang, ZC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:7页码:447-452 |
| 关键词 | Trichokirin-S1 monoclonal antibody Western blot affinity chromatography |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Prote, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,zhangzc@sunm.shcnc.ac.cn |
| 英文摘要 | Trichokirin-S1, a small ribosome-inactivating peptide recently purified from the seeds of Trichosanthes kirilowii, has potential clinical applications because of its small molecular mass. Two stable strains of hybridomas (1F11 and 2A5) that can secrete highly specific monoclonal antibodies (mAbs) against Tricholcirin-S1 have been developed using the hybridoma technique. The isotypes of these two mAbs, 1F11 and 2A5, were determined to be IgG(2a) and IgG(1), respectively. The affinity constants, which were measured by non-competitive ELISA, were found to be 2.3x10(8) M-1 and 2.8x10(8) M-1, respectively. An immunoaffinity method using 2A5-coupled Sepharose 4B was successfully developed to purify Trichokirin-S1. These two antibodies have also been used to detect Trichokirin-S1 in Western blot. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | GOURD MOMORDICA-CHARANTIA ; LUFFA-CYLINDRICA ; PROTEIN ; PURIFICATION ; MECHANISM ; INTEGRASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230665500003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1932]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, XX,Li, F,Hu, WG,et al. Preparation and preliminary application of monoclonal antibodies against Trichokirin-S1, a small ribosome-inactivating peptide from the seeds of Trichosanthes kirilowii[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(7):447-452. |
| APA | Yang, XX,Li, F,Hu, WG,Xia, HC,&Zhang, ZC.(2005).Preparation and preliminary application of monoclonal antibodies against Trichokirin-S1, a small ribosome-inactivating peptide from the seeds of Trichosanthes kirilowii.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(7),447-452. |
| MLA | Yang, XX,et al."Preparation and preliminary application of monoclonal antibodies against Trichokirin-S1, a small ribosome-inactivating peptide from the seeds of Trichosanthes kirilowii".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.7(2005):447-452. |
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