BICP0 and its RING finger domain act as ubiquitin E3 ligases in vitro
文献类型：期刊论文
| 作者 | Diao, LR; Qiao, WT; Chen, QM; Wang, C; Geng, YQ |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2005 |
| 卷号 | 50期号:7页码:636-640 |
| 关键词 | BICP0 RING finger domain ubiquitin (Ub) ubiquitin ligase E3 |
| 通讯作者 | Geng, YQ (reprint author), Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China.,cwang01@sibs.ac.cn |
| 英文摘要 | Bovine infected-cell protein 0 (BICP0) encoded by bovine herpes virus 1 (BHV-1) immediate early gene is necessary for efficient productive infection, in a large part, because it activates all 3 classes of BHV-1 genes. It also has the ability to efficiently transactivate promoters that are not derived from BHV-1. To investigate the mechanism by which BICP0 achieves these effects, we expressed and purified BICP0 and its different mutants in E. coli. In vitro assays showed that both full-length BICP0 and its isolated RING finger domain induce the accumulation of polyubiquitin chains. Mutations within the RING finger region that abolish the in vitro ubiquitination activity also cause severe reductions in BICP0 activity in other assays. Based on these, we conclude that BICP0 has the potential to act as an E3 ubiquitin ligase during viral infection and its RING finger domain is necessary for this function. These strongly support the hypothesis that BICP0 might influence virus infection through its ability to interact with the ubiquitin-proteasome pathway. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | HERPES-SIMPLEX-VIRUS ; BOVINE HERPESVIRUS-1 ; KAPPA-B ; PROTEIN ; TYPE-1 ; ICP0 ; REACTIVATION ; INFECTION ; KINASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000229823900007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1940]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Diao, LR,Qiao, WT,Chen, QM,et al. BICP0 and its RING finger domain act as ubiquitin E3 ligases in vitro[J]. CHINESE SCIENCE BULLETIN,2005,50(7):636-640. |
| APA | Diao, LR,Qiao, WT,Chen, QM,Wang, C,&Geng, YQ.(2005).BICP0 and its RING finger domain act as ubiquitin E3 ligases in vitro.CHINESE SCIENCE BULLETIN,50(7),636-640. |
| MLA | Diao, LR,et al."BICP0 and its RING finger domain act as ubiquitin E3 ligases in vitro".CHINESE SCIENCE BULLETIN 50.7(2005):636-640. |
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