Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8)
文献类型：期刊论文
| 作者 | Wang, YG; Ding, HZ; Du, P; Gan, RB; Ye, Q |
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 40期号:9页码:3068-3074 |
| 关键词 | human epidermal growth factor phoA promoter phosphate fed-batch culture pH-stat |
| 通讯作者 | Ye, Q (reprint author), E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China.,qye@ecust.edu.cn |
| 英文摘要 | Escherichia coli YK537 (pAET-8) was used to produce human epidermal growth factor (hEGF), which was expressed under the control of phoA promoter and secreted to the culture medium. In batch culture using a complex medium, phosphate was nearly depleted at 8 h, but subsequently accumulated and reached 3.8 mM. The high phosphate concentration resulted in a low hEGF expression level of 28 mg l-(1.) Hydrolysis of the organophosphorus in the complex medium by extracellular enzymes caused the accumulation of phosphate, and supply of glucose led to the consumption of the released phosphate. Feeding of glucose using a pH-stat strategy was effective in maintaining low phosphate and glucose levels, and avoided the accumulation of acetic acid. The production of hEGF reached 281 mg l(-1). When the culture was fed with the essential amino acids leucine and proline, the production of recombinant hEGF reached 332 mg l(-1). (c) 2005 Elsevier Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 关键词[WOS] | RECOMBINANT PROTEIN-PRODUCTION ; ALKALINE-PHOSPHATASE ; EFFICIENT ; PHOSPHORUS ; BACTERIA ; ACETATE ; SYSTEM ; STAT ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231841800020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1953]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, YG,Ding, HZ,Du, P,et al. Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8)[J]. PROCESS BIOCHEMISTRY,2005,40(9):3068-3074. |
| APA | Wang, YG,Ding, HZ,Du, P,Gan, RB,&Ye, Q.(2005).Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8).PROCESS BIOCHEMISTRY,40(9),3068-3074. |
| MLA | Wang, YG,et al."Production of phoA promoter-controlled human epidermal growth factor in fed-batch cultures of Escherichia coli YK537 (pAET-8)".PROCESS BIOCHEMISTRY 40.9(2005):3068-3074. |
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