Molecular cloning and functional analysis of ESGP, an embryonic stem cell and germ cell specific protein
文献类型：期刊论文
| 作者 | Chen, YM; Du, ZW; Yao, Z |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:12页码:789-796 |
| 关键词 | embryonic stem cell and germ cell specific protein (ESGP) gene embryonic stem cell Oct-4 self-renewal |
| 通讯作者 | Chen, YM (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Lab Mol & Cell Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,chenym@sibs.ac.cn ; zhongweidu@wisc.edu |
| 英文摘要 | Several putative Oct-4 downstream genes from mouse embryonic stem (ES) cells have been identified using the suppression-subtractive hybridization method. In this study, one of the novel genes encoding an ES cell and germ cell specific protein (ESGP) was cloned by rapid amplification of cDNA ends. ESGP contains 801 bp encoding an 84 amino acid small protein and has no significant homology to any known genes. There is a signal peptide at the N-terminal of ESGP protein as predicted by SeqWeb (GCG) (SeqWeb version 2.0.2, http://gcg.biosino.org:8080/). The result of immunofluorescence assay suggested that ESGP might encode a secretory protein. The expression pattern of ESGP is consistent with the expression of Oct-4 during embryonic development. ESGP protein was detected in fertilized oocyte, from 3.5 day postcoital (dpc) blastocyst to 17.5 dpc embryo, and was only detected in testis and ovary tissues in adult. In vitro, ESGP was only expressed in pluripotent cell lines, such as embryonic stem cells, embryonic carcinoma cells and embryonic germ cells, but not in their differentiated progenies. Despite its specific expression, forced expression of ESGP is not indispensable for the effect of Oct-4 on ES cell self-renewal, and does not affect the differentiation to three germ layers. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TRANSCRIPTION FACTOR ; SELF-RENEWAL ; REGULATORY ELEMENT ; MOUSE EMBRYOS ; GENE ; EXPRESSION ; OCT-4 ; DIFFERENTIATION ; STAT3 ; LINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234120300001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1963]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, YM,Du, ZW,Yao, Z. Molecular cloning and functional analysis of ESGP, an embryonic stem cell and germ cell specific protein[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(12):789-796. |
| APA | Chen, YM,Du, ZW,&Yao, Z.(2005).Molecular cloning and functional analysis of ESGP, an embryonic stem cell and germ cell specific protein.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(12),789-796. |
| MLA | Chen, YM,et al."Molecular cloning and functional analysis of ESGP, an embryonic stem cell and germ cell specific protein".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.12(2005):789-796. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。