Loss of posterior silk gland transcription specificity of fibroin light chain promoter due to absence of 41 bp sequence containing possible inhibitor binding sites
文献类型：期刊论文
| 作者 | Guo, TQ; Wang, JY; Wang, SP; Guo, XY; Huang, KW; Huang, JT; Lu, CD |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:12页码:819-825 |
| 关键词 | promoter specificity fibroin light chain silkworm Bombyx mori recombinant AcMNPV |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,cdlu@sibs.ac.cn |
| 英文摘要 | The gene encoding fibroin light chain protein (FibL) is specifically expressed in the posterior silk gland of silkworm and repressed in other tissues. The binding, sites of several transcription factors involved in the silk gland transcription specificity of fibl promoter have been recognized, including SGFB, PSGF and BMFA. Here we report the leak expression of the enhanced green fluorescent protein (EGFP) reporter gene in tissues other than the posterior silk gland in vivo when under the control of a shortened fibl promoter with deletion of the 5' terminal 41 bp sequence, which is located at -650 nt to -610 nt upstream of the fibl transcription starting site. Assay of silk gland specificity of the promoters was performed by observation of green fluorescence in tissues of silkworm larvae following inter-haemocoelic injection of recombinant Autographa californica multiple nuclear polyhedrosis, virus carrying the EGFP reporter gene controlled by different lengths of fibl promoters. Our results indicated that availability of the binding sites of several known factors, including SGFB, PSGF and BMFA, is not sufficient for intact silk gland transcription specificity of fibl promoter, and there are possible inhibitor binding sites in the 41 bp sequence (-650 nt to -610 nt) upstream of the transcription starting site which may be required to repress the activity of fibl promoter in other tissues. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | BOMBYX SERICIN-1 GENE ; HEAVY-CHAIN ; MORI ; EXPRESSION ; PROTEIN ; SYSTEM ; UPSTREAM ; CONTAINS ; DELIVERY ; ELEMENTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234120300005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1965]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, TQ,Wang, JY,Wang, SP,et al. Loss of posterior silk gland transcription specificity of fibroin light chain promoter due to absence of 41 bp sequence containing possible inhibitor binding sites[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(12):819-825. |
| APA | Guo, TQ.,Wang, JY.,Wang, SP.,Guo, XY.,Huang, KW.,...&Lu, CD.(2005).Loss of posterior silk gland transcription specificity of fibroin light chain promoter due to absence of 41 bp sequence containing possible inhibitor binding sites.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(12),819-825. |
| MLA | Guo, TQ,et al."Loss of posterior silk gland transcription specificity of fibroin light chain promoter due to absence of 41 bp sequence containing possible inhibitor binding sites".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.12(2005):819-825. |
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