Cloning and characterization of a new isoform of mouse interleukin-18
文献类型：期刊论文
| 作者 | Yang, YJ; Wang, ZY; Chen, SH; Ge, XR |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 37期号:12页码:826-834 |
| 关键词 | IL-18 isoform IL-18s IFN-gamma caspase-1 factor Xa |
| 通讯作者 | Ge, XR (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,gexirui@sibs.ac.cn |
| 英文摘要 | Interleukin-18 (IL-18) is a novel proinflammatory cytokine with potent interferon (IFN)-gamma inducing activity that plays an important biological role in the enhancement of the activity of natural killer cells and cytotoxic T lymphocytes. In this study, we have identified a novel short form of IL-18 in mouse, named IL-18s. IL-18s might be an alternative splicing variant of IL-18 and its cDNA contains a 57 bp in-frame deletion. Like IL-18, IL-18s is also widely expressed in mouse tissues. It was suggested that IL-18s might have a caspase-1-dependent mechanism for maturation and secretion similar to that of IL-18: when transfected in COS-7 cells, pro-IL-18s (22 kDa) could be detected, and the mature IL-18s (16 kDa) could also be detected when combined with caspase-1. We observed that recombinant mouse IL-18s did not show any IL-18-like function, and IL-18s could enhance the ability of IL-18 to increase IFN-gamma production by approximately 40% in mouse splenocytes. This effect was observed primarily at relative low concentrations of IL-18, suggesting that IL-18s might regulate the activity of IL-18 in the physiological conditions. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | GAMMA-INDUCING FACTOR ; GENE-EXPRESSION ; T-CELLS ; CONVERTING-ENZYME ; IMMUNE-RESPONSE ; VIRUS-INFECTION ; BINDING-PROTEIN ; IL-18 ; NK ; CYTOKINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234120300006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1966]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, YJ,Wang, ZY,Chen, SH,et al. Cloning and characterization of a new isoform of mouse interleukin-18[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(12):826-834. |
| APA | Yang, YJ,Wang, ZY,Chen, SH,&Ge, XR.(2005).Cloning and characterization of a new isoform of mouse interleukin-18.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(12),826-834. |
| MLA | Yang, YJ,et al."Cloning and characterization of a new isoform of mouse interleukin-18".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.12(2005):826-834. |
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