中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Cloning and characterization of a new isoform of mouse interleukin-18

文献类型:期刊论文

作者Yang, YJ; Wang, ZY; Chen, SH; Ge, XR
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2005
卷号37期号:12页码:826-834
关键词IL-18 isoform IL-18s IFN-gamma caspase-1 factor Xa
通讯作者Ge, XR (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,gexirui@sibs.ac.cn
英文摘要Interleukin-18 (IL-18) is a novel proinflammatory cytokine with potent interferon (IFN)-gamma inducing activity that plays an important biological role in the enhancement of the activity of natural killer cells and cytotoxic T lymphocytes. In this study, we have identified a novel short form of IL-18 in mouse, named IL-18s. IL-18s might be an alternative splicing variant of IL-18 and its cDNA contains a 57 bp in-frame deletion. Like IL-18, IL-18s is also widely expressed in mouse tissues. It was suggested that IL-18s might have a caspase-1-dependent mechanism for maturation and secretion similar to that of IL-18: when transfected in COS-7 cells, pro-IL-18s (22 kDa) could be detected, and the mature IL-18s (16 kDa) could also be detected when combined with caspase-1. We observed that recombinant mouse IL-18s did not show any IL-18-like function, and IL-18s could enhance the ability of IL-18 to increase IFN-gamma production by approximately 40% in mouse splenocytes. This effect was observed primarily at relative low concentrations of IL-18, suggesting that IL-18s might regulate the activity of IL-18 in the physiological conditions.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]GAMMA-INDUCING FACTOR ; GENE-EXPRESSION ; T-CELLS ; CONVERTING-ENZYME ; IMMUNE-RESPONSE ; VIRUS-INFECTION ; BINDING-PROTEIN ; IL-18 ; NK ; CYTOKINE
收录类别SCI
语种英语
WOS记录号WOS:000234120300006
版本出版稿
源URL[http://202.127.25.143/handle/331003/1966]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
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Yang, YJ,Wang, ZY,Chen, SH,et al. Cloning and characterization of a new isoform of mouse interleukin-18[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(12):826-834.
APA Yang, YJ,Wang, ZY,Chen, SH,&Ge, XR.(2005).Cloning and characterization of a new isoform of mouse interleukin-18.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(12),826-834.
MLA Yang, YJ,et al."Cloning and characterization of a new isoform of mouse interleukin-18".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.12(2005):826-834.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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