The human plasma proteome: Analysis of Chinese serum using shotgun strategy
文献类型：期刊论文
| 作者 | He, P; He, HZ; Dai, J; Wang, Y; Sheng, QH; Zhou, LP; Zhang, ZS; Sun, YL; Liu, F; Wang, K |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2005 |
| 卷号 | 5期号:13页码:3442-3453 |
| 关键词 | biomarkers mass spectrometry reference specimen serum proteome shotgun proteomics |
| 通讯作者 | Zhao, XH (reprint author), CAMS, Natl Lab Mol Oncol, Canc Inst & Hosp, POB 2258, Beijing, Peoples R China.,zhaoxh@pubem.cicams.ac.cn |
| 英文摘要 | We have investigated the serum proteome of Han-nationality Chinese by using shotgun strategy. A complete proteomics analysis was performed on two reference specimens from a total of 20 healthy donors, in which each sample was made from ten-pooled male or female serum, respectively. The methodology used encompassed (1) removal of six high-abundant proteins; (2) tryptic digestion of low- and high-abundant proteins of serum; (3) separation of peptide mixture by RP-HPLC followed by ESI-MS/MS identification. A total of 944 nonredundant proteins were identified under a stringent filter condition (X-corr >= 1.9, >= 2.2, and >= 3.75, Delta Cn >= 0.1, and R-sp <= 4.0) in both pooled male and female samples, in which 594 and 622 entire proteins were found, respectively. Compared with the total 3020 protein identifications confirmed by more than one laboratory or more than one specimen in HUPO Plasma Proteome Project (PPP) participating laboratories recently, 206 proteins were identified with at least two distinct peptides per protein and 185 proteins were considered as high-confidence identification. Moreover, some lower abundance serum proteins (ng/mL range) were detected, such as complement C5 and CA125, routinely used as an ovarian cancer marker in plasma and serum. The resulting nonredundant list of serum proteins would add significant information to the knowledge base of human plasma proteome and facilitate disease markers discovery. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 关键词[WOS] | 2-DIMENSIONAL ELECTROPHORESIS ; RAT SERUM ; MASS-SPECTROMETRY ; IDENTIFICATION TECHNOLOGY ; PROTEINS ; PHASE ; MODULATION ; EXPRESSION ; MAP |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231477600019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1969]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, P,He, HZ,Dai, J,et al. The human plasma proteome: Analysis of Chinese serum using shotgun strategy[J]. PROTEOMICS,2005,5(13):3442-3453. |
| APA | He, P.,He, HZ.,Dai, J.,Wang, Y.,Sheng, QH.,...&Zhao, XH.(2005).The human plasma proteome: Analysis of Chinese serum using shotgun strategy.PROTEOMICS,5(13),3442-3453. |
| MLA | He, P,et al."The human plasma proteome: Analysis of Chinese serum using shotgun strategy".PROTEOMICS 5.13(2005):3442-3453. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。