Identification of the immunogenic domains in HBsAg preS1 region using overlapping preS1 fragment fusion proteins
文献类型：期刊论文
| 作者 | Hu, WG; Wei, J; Xia, HC; Yang, XX; Li, F; Li, GD; Wang, Y; Zhang, ZC |
| 刊名 | WORLD JOURNAL OF GASTROENTEROLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 11期号:14页码:2088-2094 |
| 关键词 | HBV preS1 GST fusion protein Immunogenic domain Overlapping |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Prote, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,zhangzc@sunm.shcnc.ac.cn |
| 英文摘要 | AIM: The incorporation of hepatitis B virus (HBV) preS1 region into epitope-based vaccines against HBV has been accepted widely, but the incorporate site and size of preS1 sequence is controversial. Therefore our purpose was to further investigate its immunogenic domains for the epitope-based hepatitis B vaccine design. METHODS: Eight GST fusion proteins containing overlapping preS1 fragments in preS1 (21-119) region were expressed in E. coli. Using these purified fusion proteins, the immunogenic domains in preS1 region were identified in detail in mice and humans by Western blot analysis and ELISA. RESULTS: The results in mice showed that the immunogenic domains mainly existed in preS1 (21-59) and preS1 (95-109). Similarly, these fragments had strong immunogenicity in humans; whereas the other parts except for preS1 (60-70) also had some immunogenicity. More importantly, a major immunogenic domain, preS1 (34-59), which has much stronger immunogenicity, was identified. Additionally, the antibodies against some preS1 fragments, especially preS1 (34-59), were speculated to be virus-neutralizing. CONCLUSION: Eight GST fusion proteins containing overlapping preS1 fragments were prepared successfully. They were used for the study on the immunogenic domains in preS1 (21-119) region. The preS1 (34-59) fragments were the major immunogenic domains in the preS1 region, and the antibodies against these fragments were speculated to be virus-neutralizing. Therefore, the incorporation of preS1 (34-59) fragments into epitope-based HBV vaccines may be efficient for enhancement of immune response. Additionally, the results also imply that there are more complex immune responses to preS1 region and more abundant immunogenic domains in humans. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved. |
| 学科主题 | Gastroenterology & Hepatology |
| 类目[WOS] | Gastroenterology & Hepatology |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000208102600007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1973]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, WG,Wei, J,Xia, HC,et al. Identification of the immunogenic domains in HBsAg preS1 region using overlapping preS1 fragment fusion proteins[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2005,11(14):2088-2094. |
| APA | Hu, WG.,Wei, J.,Xia, HC.,Yang, XX.,Li, F.,...&Zhang, ZC.(2005).Identification of the immunogenic domains in HBsAg preS1 region using overlapping preS1 fragment fusion proteins.WORLD JOURNAL OF GASTROENTEROLOGY,11(14),2088-2094. |
| MLA | Hu, WG,et al."Identification of the immunogenic domains in HBsAg preS1 region using overlapping preS1 fragment fusion proteins".WORLD JOURNAL OF GASTROENTEROLOGY 11.14(2005):2088-2094. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。