The effect of isoforms of the cell polarity protein, human ASIP, on the cell cycle and Fas/FasL-mediated apoptosis in human hepatoma cells
文献类型：期刊论文
| 作者 | Hu, Y; Fang, C; Xu, Y |
| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
 |
| 出版日期 | 2005 |
| 卷号 | 62期号:17页码:1974-1983 |
| 关键词 | hASIP isoform cell cycle FasL apoptosis BEL-7404 human hepatoma cell |
| 通讯作者 | Xu, Y (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol & Cellular Oncol, 320 YueYang Rd, Shanghai 200031, Peoples R China.,yhxu@sibs.ac.cn |
| 英文摘要 | Human ASIP (hASIP) is expressed as numerous alternative splicing isoforms and there is an atypical protein kinease C (aPKC) phosphorylation site in exon 17b of the encoded sequence. We have identified an important role for exon 17b in cancer cells. Our results showed that hASIP-sa and sb had different effects on cell growth and Fas/FasL-mediated apoptosis in BEL-7404 human hepatoma cells. Human ASIP-sa modified the S phase of the cell cycle and might stimulate cell proliferation. Growth inhibition by hASIP-a antisense oligonucleotide-confirmed the positive action of hASIP-sa. Compared with hASIP-sa, hASIP-sb accelerated Fas/FasL-induced apoptosis, examined by sub-G1 accumulation, chromatin condensation, nuclear fragmentation, PARP cleavage, caspase-8 degradation and mitochondria-regulated cell death. Treatment with aPKC inhibitor could enhance Fas/FasL-mediated apoptosis in hASIP-sa-overexpressing cells, suggesting that hASIP-sa and its interaction with aPKC might contribute to the malignant growth and the blocking of Fas/FasL-mediated apoptosis, while hASIP-sb might function as an antagonist of hASIP-sa. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | EPITHELIAL TIGHT JUNCTION ; ADHESION MOLECULE JAM ; KINASE-C ; TRANSCRIPTION FACTOR ; SPLICED ISOFORM ; PAR-3 ; COMPLEX ; ELEGANS ; DEATH ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000232497400007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1978]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, Y,Fang, C,Xu, Y. The effect of isoforms of the cell polarity protein, human ASIP, on the cell cycle and Fas/FasL-mediated apoptosis in human hepatoma cells[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2005,62(17):1974-1983. |
| APA | Hu, Y,Fang, C,&Xu, Y.(2005).The effect of isoforms of the cell polarity protein, human ASIP, on the cell cycle and Fas/FasL-mediated apoptosis in human hepatoma cells.CELLULAR AND MOLECULAR LIFE SCIENCES,62(17),1974-1983. |
| MLA | Hu, Y,et al."The effect of isoforms of the cell polarity protein, human ASIP, on the cell cycle and Fas/FasL-mediated apoptosis in human hepatoma cells".CELLULAR AND MOLECULAR LIFE SCIENCES 62.17(2005):1974-1983. |
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