Proteomic analysis with integrated multiple dimensional liquid chromatography/mass spectrometry based on elution of ion exchange column using pH steps
文献类型：期刊论文
| 作者 | Dai, J; Shieh, CH; Sheng, QH; Zhou, H; Zeng, R |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 77期号:18页码:5793-5799 |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Res Ctr Proteome Anal, Key Lab Prote, Inst Biochem & Cell Biol,Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn |
| 英文摘要 | A novel integrated multidimensional liquid chromatography (IMDL) method is demonstrated for the separation of peptide mixtures by two-dimensional HPLC coupled with ion trap mass spectrometry. The method uses an integrated column, containing both strong cation exchange and reversed-phase sections for two-dimensional liquid chromatography. The peptide mixture was fractionated by a pH step using a series of pH buffers, followed by reversed-phase chromatography. Since no salt was used during separation, the integrated multidimensional liquid chromatography can be directly connected to mass spectrometry for peptide analysis. The pH buffers were injected from an autosampler, and the entire process can be carried out on a one-dimensional liquid chromatography system. In a single analysis, the IMDL system, coupled with linear ion trap mass spectrometry, identified more than 2000 proteins in mouse liver. The peptides were eluted according to their pi distribution. The resolution of the pH fractionation is similar to 0.5 pH unit. The method has low overlapping across pH fractions, good resolution of peptide mixture, and good correlation of peptide pIs with pH steps. This method provides a technique for large-scale protein identification using existing one-dimensional HPLC systems. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Analytical |
| 关键词[WOS] | TANDEM MASS-SPECTROMETRY ; PROTEIN IDENTIFICATION ; YEAST PROTEOME ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231919400003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1981]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Dai, J,Shieh, CH,Sheng, QH,et al. Proteomic analysis with integrated multiple dimensional liquid chromatography/mass spectrometry based on elution of ion exchange column using pH steps[J]. ANALYTICAL CHEMISTRY,2005,77(18):5793-5799. |
| APA | Dai, J,Shieh, CH,Sheng, QH,Zhou, H,&Zeng, R.(2005).Proteomic analysis with integrated multiple dimensional liquid chromatography/mass spectrometry based on elution of ion exchange column using pH steps.ANALYTICAL CHEMISTRY,77(18),5793-5799. |
| MLA | Dai, J,et al."Proteomic analysis with integrated multiple dimensional liquid chromatography/mass spectrometry based on elution of ion exchange column using pH steps".ANALYTICAL CHEMISTRY 77.18(2005):5793-5799. |
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