Two-dimensional gel electrophoresis maps of the proteome and phosphoproteome of primitively cultured rat mesangial cells
文献类型：期刊论文
| 作者 | Jiang, XS; Tang, LY; Cao, XJ; Zhou, H; Xia, QC; Wu, JR; Zeng, R |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2005 |
| 卷号 | 26期号:23页码:4540-4562 |
| 关键词 | mass spectrometry mesangial cell phosphoproteome proteome two-dimensional electrophoresis |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Res Ctr Proteome Anal,Key Lab Proteom,Grad Sch, 320 YueYang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn |
| 英文摘要 | Mesangial cells (MC) play an important role in maintaining the structure and function of the glomerulus. The proliferation of MC is a prominent feature of many kinds of glomerular disease. The first reference 2-DE maps of rat mesangial cells (RMC), stained with silver staining or Pro-Q Diamond dye, have been established here to describe the proteome and phosphoproteome of RMC, respectively. A total of 157 selected protein spots, corresponding to 118 unique proteins, have been identified by MALDI-TOF-MS or LC-ESI-IT-MS/MS, in which 37 protein spots representing 28 unique proteins have also been stained with Pro-Q Diamond, indicating that they are in phosphorylated forms. All the identified proteins were bioinformatically annotated in detail according to their physiochemical characteristics, subcellular location, and function. Most of the separated or identified protein spots are distributed in the area of mass 10-70 kDa and p/5.0-8.0. The identified proteins include mainly cytoplasmic and nuclear proteins and some mitochondrial, endoplasmic reticulum, and membrane proteins. These proteins are classified into different functional groups such as structure and mobility proteins (21.2%), metabolic enzymes (16.9%), protein folding and metabolism proteins (13.6%), signaling proteins (14.4%), heat-shock proteins (7.6%), and other functional proteins (12.7%). While structure and mobility proteins are mostly represented by protein spots with high abundance, signaling proteins are mostly represented by protein spots with relatively low abundance. Such a 2-DE database for RMC, especially with many signaling proteins and phosphoproteins characterized, will provide a valuable resource for comparative proteomics analysis of normal and pathologic conditions affecting MC function or pathologic progress. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 关键词[WOS] | SUBCELLULAR-LOCALIZATION ; ENDOPLASMIC-RETICULUM ; MASS-SPECTROMETRY ; KINASE-A ; PHOSPHORYLATION ; PROTEINS ; IDENTIFICATION ; APOPTOSIS ; COFILIN ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234066800017 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1985]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, XS,Tang, LY,Cao, XJ,et al. Two-dimensional gel electrophoresis maps of the proteome and phosphoproteome of primitively cultured rat mesangial cells[J]. ELECTROPHORESIS,2005,26(23):4540-4562. |
| APA | Jiang, XS.,Tang, LY.,Cao, XJ.,Zhou, H.,Xia, QC.,...&Zeng, R.(2005).Two-dimensional gel electrophoresis maps of the proteome and phosphoproteome of primitively cultured rat mesangial cells.ELECTROPHORESIS,26(23),4540-4562. |
| MLA | Jiang, XS,et al."Two-dimensional gel electrophoresis maps of the proteome and phosphoproteome of primitively cultured rat mesangial cells".ELECTROPHORESIS 26.23(2005):4540-4562. |
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