Large-scale identification of human biliary proteins from a cholesterol stone patient using a proteomic approach
文献类型：期刊论文
| 作者 | Zhou, H; Chen, B; Li, RX; Sheng, QH; Li, SJ; Zhang, L; Li, L; Xia, QC; Wang, HY; Zeng, R |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2005 |
| 卷号 | 19期号:23页码:3569-3578 |
| 通讯作者 | Wang, HY (reprint author), Eastern Hepatobiliary Surg Hosp, 225 Changhai Rd, Shanghai 200438, Peoples R China.,hywangk@online.sh.cn ; zr@sibs.ac.cn |
| 英文摘要 | Gallbladder bile, one of the most important body fluids, is composed of water, inorganic ions, conjugated bile salts, phospholipids, cholesterol, bilirubin, mucin and proteins. The separation and identification of bile proteins remain difficult due to the complexity of this matrix. In the present study, human gallbladder bile was obtained from a cholesterol stone patient, and the proteins were isolated and purified by dialysis, precipitation and delipidation procedures. The resulting proteins were divided into several aliquots. One aliquot was subjected to two-dimensional gel electrophoresis (2DE). The protein spots were then in-gel digested and analyzed by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Another aliquot was directly digested and analyzed by a combination of strong cation-exchange (SCX) and reversed-phase (RP) chromatography prior to tandem mass spectrometry (2D-LC/MS/MS). Eventually, 48 and 218 unique proteins were identified from 2DE/MS and 2D-LC/MS/MS, respectively, resulting in a total of 222 unique identified proteins. Of the 218 proteins identified by 2D-LC/MS/MS, 92 were identified based on more than one unique tryptic peptide, and, of the total 222 proteins, 98 were identified based on more than one unique tryptic peptide. Copyright (c) 2005 John Wiley & Sons, Ltd. |
| 学科主题 | Chemistry; Spectroscopy |
| 类目[WOS] | Chemistry, Analytical ; Spectroscopy |
| 关键词[WOS] | 2-DIMENSIONAL GEL-ELECTROPHORESIS ; HUMAN GALLBLADDER BILE ; MASS-SPECTROMETRY ; POLYACRYLAMIDE-GELS ; CEREBROSPINAL-FLUID ; SHOTGUN PROTEOMICS ; HUMAN HEPATOMA ; NUCLEATION ; RESOLUTION ; COMPLEX |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000233715900020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1986]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, H,Chen, B,Li, RX,et al. Large-scale identification of human biliary proteins from a cholesterol stone patient using a proteomic approach[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2005,19(23):3569-3578. |
| APA | Zhou, H.,Chen, B.,Li, RX.,Sheng, QH.,Li, SJ.,...&Zeng, R.(2005).Large-scale identification of human biliary proteins from a cholesterol stone patient using a proteomic approach.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,19(23),3569-3578. |
| MLA | Zhou, H,et al."Large-scale identification of human biliary proteins from a cholesterol stone patient using a proteomic approach".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 19.23(2005):3569-3578. |
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