Detecting AFP mRIVA in peripheral blood of the patients with hepatocellular carcinoma, liver cirrhosis and hepatitis
文献类型：期刊论文
| 作者 | Yao, F; Guo, JM; Xu, CF; Lou, YL; Xiao, BX; Zhou, WH; Chen, J; Hu, YR; Liu, Z; Hong, GF |
| 刊名 | CLINICA CHIMICA ACTA
 |
| 出版日期 | 2005 |
| 卷号 | 361期号:1页码:119-127 |
| 关键词 | AFP mRNA hepatocellular carcinoma liver cirrhosis hepatitis peripheral blood immunomagnetic bead |
| 通讯作者 | Guo, JM (reprint author), Ningbo Univ, Sch Med, Ningbo 315211, Zhejiang, Peoples R China.,junmingguo@yahoo.com |
| 英文摘要 | Background: The low frequency of disseminated carcinoma cells in the blood now makes immunomagnetic bead sorting and reverse transcriptase-polymerase chain reaction (RT-PCR) technique more popular. Methods: Three milliliters of peripheral blood were collected from 91 patients and 18 normal donors. The circulating carcinoma cells were enriched with CD45 and Ber-EP4 immunomagnetic beads. The a-fetoprotein (AFP) mRNA was amplified with nested RT-PCR. Results: The total positive detection rate was 72.1%, 43.8%, 25.0%, 100%, and 66.7% in patients with hepatocellular carcinoma (HCC) untreated, liver cirrhosis (LC), hepatitis, metastasis liver cancer, and postsurgery of hepatocellular carcinoma, respectively. There was a significant difference among the patients with HCC, LC and hepatitis (HCC vs. LC, P<0.05; HCC vs. hepatitis. P<0.01) and between Class A and B of the HCC patients (P<0.05). Meanwhile, AFP mRNA was markedly expressed in HCC patients compared to the patients with no HCC (LC and hepatitis). The levels of aspartate transaminase (AST) and gamma-glutamyltranspeptidase (GGT) were significantly different in AFP mRNA-positive patients with autoimmune chronic active hepatitis B (CAHB) or LC in contrast to the corresponding negative patients. Conclusion: Combining negative and positive immunomagnetic bead sorting and RT-PCR technique can effectively detect circulating tumor cells. AFP mRNA is a more reliable marker of metastasis compared to serum AFP. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Medical Laboratory Technology |
| 类目[WOS] | Medical Laboratory Technology |
| 关键词[WOS] | FETOPROTEIN MESSENGER-RNA ; CIRCULATING TUMOR-CELLS ; BREAST-CANCER PATIENTS ; B-VIRUS ; BONE-MARROW ; IMMUNOMAGNETIC SEPARATION ; UNITED-STATES ; RECURRENCE ; ENRICHMENT ; RESECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000232965600011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1994]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yao, F,Guo, JM,Xu, CF,et al. Detecting AFP mRIVA in peripheral blood of the patients with hepatocellular carcinoma, liver cirrhosis and hepatitis[J]. CLINICA CHIMICA ACTA,2005,361(1):119-127. |
| APA | Yao, F.,Guo, JM.,Xu, CF.,Lou, YL.,Xiao, BX.,...&Hong, GF.(2005).Detecting AFP mRIVA in peripheral blood of the patients with hepatocellular carcinoma, liver cirrhosis and hepatitis.CLINICA CHIMICA ACTA,361(1),119-127. |
| MLA | Yao, F,et al."Detecting AFP mRIVA in peripheral blood of the patients with hepatocellular carcinoma, liver cirrhosis and hepatitis".CLINICA CHIMICA ACTA 361.1(2005):119-127. |
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