Use of different carbon sources in cultivation of recombinant Pichia pastoris for angiostatin production
文献类型：期刊论文
| 作者 | Xie, JL; Zhou, QW; Peng, D; Gan, RB; Qin, Y |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2005 |
| 卷号 | 36期号:1页码:210-216 |
| 关键词 | Pichia pastoris angiostatin lactic acid fed-batch culture |
| 通讯作者 | Qin, Y (reprint author), E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China.,qye@ecust.edu.cn |
| 英文摘要 | To improve the growth of recombinant Pichia pastoris with a phenotype of Mut(S) and expression of angiostatin, the effects of glycerol, sorbitol, acetate and lactic acid which were, respectively, added together with methanol in the expression phase, were studied in a 5-l fermentor. Methanol concentration was automatically controlled at 5 g/l by a methanol monitor and control system, while the feeding of the other carbon source was manually adjusted. The angiostatin production level was 108 mg/l when glycerol was added at an initial rate of 2.3 g/h and gradually increased to 9.9 g/h within an induction period of 96 h. The angiostatin concentration was 141 mg/l as sorbitol was used, while only 52 m-A were obtained on acetate. The highest angiostatin production of 191 mg/l was achieved as lactic acid was used; whose feeding rate was gradually increased from 2.6 to 11.3 g/h. Lactic acid accumulated during the induction phase and reached 6.3 g/l at the end of fermentation. However, the accumulation of lactic acid did not interfere with angiostatin production, indicating that lactic acid to be a non-repressive carbon source. The average productivity and specific productivity of angiostatin obtained on lactic acid and methanol were, respectively, 2.96 and 0.044 mg/(g h), 1.7- and 2.5-fold of those obtained in the fermentation fed with glycerol and methanol. (C) 2004 Elsevier Inc. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | FED-BATCH FERMENTATION ; PROTEIN EXPRESSION ; YEAST ; GENE ; STRATEGIES ; SECRETION ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000226715400008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1995]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xie, JL,Zhou, QW,Peng, D,et al. Use of different carbon sources in cultivation of recombinant Pichia pastoris for angiostatin production[J]. ENZYME AND MICROBIAL TECHNOLOGY,2005,36(1):210-216. |
| APA | Xie, JL,Zhou, QW,Peng, D,Gan, RB,&Qin, Y.(2005).Use of different carbon sources in cultivation of recombinant Pichia pastoris for angiostatin production.ENZYME AND MICROBIAL TECHNOLOGY,36(1),210-216. |
| MLA | Xie, JL,et al."Use of different carbon sources in cultivation of recombinant Pichia pastoris for angiostatin production".ENZYME AND MICROBIAL TECHNOLOGY 36.1(2005):210-216. |
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