Antibody responses to individual proteins of SARS coronavirus and their neutralization activities
文献类型：期刊论文
| 作者 | Qiu, MF; Shi, YL; Guo, ZB; Chen, ZL; He, RQ; Chen, RS; Zhou, DS; Dai, EH; Wang, XY; Si, BY |
| 刊名 | MICROBES AND INFECTION
 |
| 出版日期 | 2005 |
| 卷号 | 7期号:1页码:882-889 |
| 关键词 | SARS coronavirus recombinant protein protein microarray antibodies neutralizing antibodies |
| 通讯作者 | Yang, RF (reprint author), Acad Mil Med Sci, Inst Microbiol & Epidemiol, 20 Dongdajie, Beijing 100071, Peoples R China.,yangrf@nic.bmi.ac.cn ; yangrf@nic.bmi.ac.cn |
| 英文摘要 | A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle. (c) 2005 Elsevier SAS. All rights reserved. |
| 学科主题 | Immunology; Infectious Diseases; Microbiology |
| 类目[WOS] | Immunology ; Infectious Diseases ; Microbiology |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; SPIKE PROTEIN ; NUCLEOCAPSID PROTEIN ; ARRAYS ; VIRUS ; IDENTIFICATION ; SERODIAGNOSIS ; MICROARRAYS ; SEQUENCE ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230224400010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1996]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Qiu, MF,Shi, YL,Guo, ZB,et al. Antibody responses to individual proteins of SARS coronavirus and their neutralization activities[J]. MICROBES AND INFECTION,2005,7(1):882-889. |
| APA | Qiu, MF.,Shi, YL.,Guo, ZB.,Chen, ZL.,He, RQ.,...&Yang, RF.(2005).Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.MICROBES AND INFECTION,7(1),882-889. |
| MLA | Qiu, MF,et al."Antibody responses to individual proteins of SARS coronavirus and their neutralization activities".MICROBES AND INFECTION 7.1(2005):882-889. |
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