In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS
文献类型：期刊论文
| 作者 | Luo, HB; Ye, F; Sun, T; Yue, LD; Peng, SY; Chen, J; Li, GW; Du, Y; Xie, YH; Yang, YM |
| 刊名 | BIOPHYSICAL CHEMISTRY
 |
| 出版日期 | 2004 |
| 卷号 | 112期号:1页码:15-25 |
| 关键词 | SARS nucleocapsid protein SPR circular dichmism fluorescence protein folding and unfolding |
| 通讯作者 | Shen, X (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Drug Discovery & Design Ct, Shanghai 201203, Peoples R China.,xshen@mail.shcnc.ac.cn ; hljiang@mail.shcnc.ac.cn |
| 英文摘要 | The major biochemical and thermodynamic features of nucelocapsid protein of SARS coronavirus (SARS-NP) were characterized by use of non-denatured gel electrophoresis, size-exclusion chromatographic and surface plasmon resonance (SPR) techniques. The results showed that SARS_NP existed in vitro as oligomer, more probably dimer, as the basic functional unit. This protein shows its maximum conformational stability near pH 9.0, and it seems that its oligomer dissociation and protein unfolding occur simultaneously. Thermal-induced unfolding for SARS_NP was totally irreversible. Both the thermal and chemical denaturant-induced denaturation analyses showed that oligomeric SARS_NP unfolds and refolds through a two-state model, and the electrostatic interactions among the charge groups of SARS_NP made a significant contribution to its conformational stability. (C) 2004 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Chemistry |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; MOUSE HEPATITIS-VIRUS ; ENVELOPE PROTEIN ; ESCHERICHIA-COLI ; P-PROTEIN ; CORONAVIRUS ; RNA ; STABILITY ; IDENTIFICATION ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000224852300003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2005]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Luo, HB,Ye, F,Sun, T,et al. In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS[J]. BIOPHYSICAL CHEMISTRY,2004,112(1):15-25. |
| APA | Luo, HB.,Ye, F.,Sun, T.,Yue, LD.,Peng, SY.,...&Jiang, HL.(2004).In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS.BIOPHYSICAL CHEMISTRY,112(1),15-25. |
| MLA | Luo, HB,et al."In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS".BIOPHYSICAL CHEMISTRY 112.1(2004):15-25. |
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