Identification of the role of a cysteine-rich region of PC6B by determining the enzymatic characteristics of its mutants
文献类型：期刊论文
| 作者 | Wang, L; Yang, GZ; Wu, XF |
| 刊名 | MOLECULAR BIOTECHNOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 27期号:1页码:15-22 |
| 关键词 | mPC6B Tn-5B1-4 insect cells Cys-rich motif baculovirus |
| 通讯作者 | Wu, XF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,xfwu@sunm.shcnc.ac.cn |
| 英文摘要 | In the proprotein convertases family, mouse PC6B (mPC6B) has a very large cysteine-rich region (CRR), consisting of 22 tandem cysteine-rich (Cys-rich) repeated segments. The role of this region remains elusive. In this report, to get insight on the possible role of the CRR, we constructed four truncated mPC6B mutant genes with 0, 5, 11, and 22 Cys-rich repeated segments remaining; using the baculovirsu-expression system and a simple purification method, we obtained four enzyme mutants of mPC6B. By determining their optimal pH and calcium ion concentration for enzymatic activity and their thermal stability, we found that CRR did not affect pH optimum and Ca2+ optimum compared with the p-domain. However, CRR acted as a stabilizing domain in addition to the p-domain. By kinetic analyses of four mutants, we found that the long Cys-rich repeats in the native form of mPC6B reduced its V-max. These facts suggest that CRR acts as an important part of functional domain. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | SUBTILISIN-LIKE PROTEASE ; KEX2-LIKE PROCESSING ENDOPROTEASE ; PROHORMONE CONVERTASES ; FUNCTIONAL EXPRESSION ; YEAST KEX2 ; CLEAVAGE ; ACTIVATION ; GENE ; CLONING ; FAMILY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221688800003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2013]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, L,Yang, GZ,Wu, XF. Identification of the role of a cysteine-rich region of PC6B by determining the enzymatic characteristics of its mutants[J]. MOLECULAR BIOTECHNOLOGY,2004,27(1):15-22. |
| APA | Wang, L,Yang, GZ,&Wu, XF.(2004).Identification of the role of a cysteine-rich region of PC6B by determining the enzymatic characteristics of its mutants.MOLECULAR BIOTECHNOLOGY,27(1),15-22. |
| MLA | Wang, L,et al."Identification of the role of a cysteine-rich region of PC6B by determining the enzymatic characteristics of its mutants".MOLECULAR BIOTECHNOLOGY 27.1(2004):15-22. |
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