Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1)
文献类型：期刊论文
| 作者 | Hu, JH; Zhang, JF; Ma, YH; Jiang, J; Yang, N; Li, XB; Chi, ZGY; Fei, J; Guo, LH |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2004 |
| 卷号 | 14期号:1页码:54-59 |
| 关键词 | GABA GAT1 testes sperm reproduction transgenic |
| 通讯作者 | Fei, J (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,mhzhang@sunm.shcne.ac.cn |
| 英文摘要 | It is well documented that gamma-aminobutyric acid (GABA) system existed in reproductive organs. Recent researches showed that GABA(A) and GABA(B) receptors were present in testis and sperm, and might mediate the acrosome reaction induced by GABA and progesterone. GABA transporter I (GAT1) also existed in testis and sperm, but its physiological function was unknown. In the present study, we used GAT1 overexpressing mice to explore GAT1 function in male reproductive system. We found that the expression level of GAT1 continuously increased in wild-type mouse testis from 1 month to 2 months after birth. GAT1 overexpression in mouse affected testis development, which embodied reduced testis mass and slowed spermatogenesis in transgenic mice. Moreover, transgenic mice showed increase of the percentage of broken sperm. The further study revealed that the reproductive capacity was impaired in GAT1 overexpressing mice. In addition, testosterone level was significantly low in transgenic mice compared with that in wild-type mice. Our findings provided the first evidence that abnormal expression of GAT1 could result in dysgenesis, and indicated that GAT1 might be therapeutically targeted for contraception or dysgenesis treatment. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | SPERM ACROSOME REACTION ; HUMAN SPERMATOZOA ; GABA TRANSPORTER ; RAT TESTIS ; RECEPTOR ; BRAIN ; IDENTIFICATION ; PROGESTERONE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220195100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2016]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, JH,Zhang, JF,Ma, YH,et al. Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1)[J]. CELL RESEARCH,2004,14(1):54-59. |
| APA | Hu, JH.,Zhang, JF.,Ma, YH.,Jiang, J.,Yang, N.,...&Guo, LH.(2004).Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1).CELL RESEARCH,14(1),54-59. |
| MLA | Hu, JH,et al."Impaired reproduction in transgenic mice overexpressing gamma-aminobutyric acid transporter I (GAT1)".CELL RESEARCH 14.1(2004):54-59. |
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