Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells
文献类型：期刊论文
| 作者 | Wang, L; Yang, GZ; Wu, XF |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2004 |
| 卷号 | 11期号:1页码:63-69 |
| 关键词 | BTMD-mPC6B Tn-5B1-4 insect cells |
| 通讯作者 | Wu, XF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,xfwu@sumn.shcnc.ac.cn |
| 英文摘要 | we constructed a mouse PC6B truncated mutant and introduced a tag of 6 consecutive histidines at its carboxyl terminus for simple purification. Using the baculovirus expression system and standard enzymatic assay, we obtained recombinant mouse PC6B protein and with enzymatic activity. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SUBTILISIN-LIKE PROTEASE ; KEX2-LIKE PROCESSING ENDOPROTEASE ; PROHORMONE CONVERTASES ; YEAST KEX2 ; IDENTIFICATION ; CLEAVAGE ; ACTIVATION ; GENE ; FAMILY ; REGION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220210400010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2018]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, L,Yang, GZ,Wu, XF. Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells[J]. PROTEIN AND PEPTIDE LETTERS,2004,11(1):63-69. |
| APA | Wang, L,Yang, GZ,&Wu, XF.(2004).Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells.PROTEIN AND PEPTIDE LETTERS,11(1),63-69. |
| MLA | Wang, L,et al."Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells".PROTEIN AND PEPTIDE LETTERS 11.1(2004):63-69. |
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