A flexible peptide linker enhances the immunoreactivity of two copies HBsAg preS1 (21-47) fusion protein
文献类型：期刊论文
| 作者 | Hu, WG; Li, F; Yang, XX; Li, Z; Xia, HC; Li, GD; Wang, Y; Zhang, ZC |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 107期号:1页码:83-90 |
| 关键词 | hepatitis B virus (HBV) preS1 peptide E. coli expression GST fusion protein flexible peptide linker anti-preS1 (21-47) antibody |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,zhangzc@sunm.shcnc.ac.cn |
| 英文摘要 | PreS1 (21-47) region of HBV large surface protein is hepatocyte receptor binding site and the anti-preS 1 (21-47) antibody possesses the virus-neutralizing activity and protective effect. It is important to obtain the peptide with higher immunoreactivity on a large scale for detecting the anti-preSl (21-47) antibody in the sera from HBV infected patients and future vaccine recipients. The expression vector pGEX SLS, which expressed two copies of the preS 1 (21-47) peptide connected by a flexible linker (Gly(4)Ser)(3) fused to glutathione S-transferase (GST), was constructed. The fusion protein, named GST-SLS, was highly expressed in E. coli and purified by affinity chromatography. Ninety milligrams purified protein can be obtained from 11 of culture. The data in ELISA analysis showed that the immunoreactivity of GST-SLS was enhanced significantly in comparison with GST-S 11, a GST fusion protein with two copies preS 1 (21-47) linked directly; GST-S 1, another GST fusion protein with one copy preS 1 (21-47) and preS 1 (21-47) synthesized peptide. In addition, GST-SLS has been tried to use in detecting anti-preS 1 (21-47) antibody in the sera from HBV infected patients and a satisfied result was gained. Therefore, GST-SLS may have potential to be developed into a new kit for diagnosis and prognosis of hepatitis B (HB) patients. (C) 2003 Elsevier B.V. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | HEPATITIS-B-VIRUS ; ESCHERICHIA-COLI ; ANTIBODY ; INFECTION ; RESPONSES ; RECOVERY ; ANTIGEN ; LIVER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187912200008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2020]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, WG,Li, F,Yang, XX,et al. A flexible peptide linker enhances the immunoreactivity of two copies HBsAg preS1 (21-47) fusion protein[J]. JOURNAL OF BIOTECHNOLOGY,2004,107(1):83-90. |
| APA | Hu, WG.,Li, F.,Yang, XX.,Li, Z.,Xia, HC.,...&Zhang, ZC.(2004).A flexible peptide linker enhances the immunoreactivity of two copies HBsAg preS1 (21-47) fusion protein.JOURNAL OF BIOTECHNOLOGY,107(1),83-90. |
| MLA | Hu, WG,et al."A flexible peptide linker enhances the immunoreactivity of two copies HBsAg preS1 (21-47) fusion protein".JOURNAL OF BIOTECHNOLOGY 107.1(2004):83-90. |
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