Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase
文献类型:期刊论文
作者 | Yao, YN; Zhang, QS; Yan, XZ; Zhu, G; Wang, ED |
刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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出版日期 | 2004 |
卷号 | 313期号:1页码:129-134 |
关键词 | aminoacyl-tRNA synthetase arginine tRNA argU comparative modeling ArgRS |
通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
英文摘要 | Previous investigations show that tRNA(Arg)-induced conformational changes of arginyl-tRNA synthetase (ArgRS) Omega-loop region (Escherichia coli (E coli), Ala451-Ala457) may contribute to the productive conformation of the enzyme catalytic core, and E. coli tRNA(2)(Arg)(ICG)-bound and -free conformations of the Omega-loop exchange at an intermediate rate on NMR timescale. Herein, we report that E. coli ArgRS catalyzes tRNA(2)(Arg)(ICG) and tRNA(4)(Arg)(UCU) with similar efficiencies. However, F-19 NMR spectroscopy of 4-fluorotryptophan-labeled E. coli ArgRS reveals that the tRNA(4)(Arg)(UCU)-bound and -free conformations of the Omega-loop region interconvert very slowly and the lifetime of bound conformation is much longer than 0.33ms. Therefore, tRNA(4)(Arg) (UCU) differs from tRNA(2)(Arg)(ICG) in the conformation-exchanging rate of the Omega-loop. Comparative structure model of E. coli ArgRS is presented to rationalize these F-19 NMR data. Our F-19 NMR and catalytic assay results suggest that the tRNA(Arg)-induced conformational changes of Omega-loop little contribute to the productive conformation of ArgRS catalytic core. (C) 2003 Elsevier Inc. All rights reserved. |
学科主题 | Biochemistry & Molecular Biology; Biophysics |
类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
关键词[WOS] | PROTEIN-STRUCTURE ; F-19 NMR ; GENES ; PURIFICATION ; RECOGNITION ; EXPRESSION ; IDENTITY |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000187745900021 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/2021] ![]() |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Yao, YN,Zhang, QS,Yan, XZ,et al. Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,313(1):129-134. |
APA | Yao, YN,Zhang, QS,Yan, XZ,Zhu, G,&Wang, ED.(2004).Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,313(1),129-134. |
MLA | Yao, YN,et al."Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 313.1(2004):129-134. |
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