中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase

文献类型:期刊论文

作者Yao, YN; Zhang, QS; Yan, XZ; Zhu, G; Wang, ED
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2004
卷号313期号:1页码:129-134
关键词aminoacyl-tRNA synthetase arginine tRNA argU comparative modeling ArgRS
通讯作者Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,
英文摘要Previous investigations show that tRNA(Arg)-induced conformational changes of arginyl-tRNA synthetase (ArgRS) Omega-loop region (Escherichia coli (E coli), Ala451-Ala457) may contribute to the productive conformation of the enzyme catalytic core, and E. coli tRNA(2)(Arg)(ICG)-bound and -free conformations of the Omega-loop exchange at an intermediate rate on NMR timescale. Herein, we report that E. coli ArgRS catalyzes tRNA(2)(Arg)(ICG) and tRNA(4)(Arg)(UCU) with similar efficiencies. However, F-19 NMR spectroscopy of 4-fluorotryptophan-labeled E. coli ArgRS reveals that the tRNA(4)(Arg)(UCU)-bound and -free conformations of the Omega-loop region interconvert very slowly and the lifetime of bound conformation is much longer than 0.33ms. Therefore, tRNA(4)(Arg) (UCU) differs from tRNA(2)(Arg)(ICG) in the conformation-exchanging rate of the Omega-loop. Comparative structure model of E. coli ArgRS is presented to rationalize these F-19 NMR data. Our F-19 NMR and catalytic assay results suggest that the tRNA(Arg)-induced conformational changes of Omega-loop little contribute to the productive conformation of ArgRS catalytic core. (C) 2003 Elsevier Inc. All rights reserved.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]PROTEIN-STRUCTURE ; F-19 NMR ; GENES ; PURIFICATION ; RECOGNITION ; EXPRESSION ; IDENTITY
收录类别SCI
语种英语
WOS记录号WOS:000187745900021
版本出版稿
源URL[http://202.127.25.143/handle/331003/2021]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
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GB/T 7714
Yao, YN,Zhang, QS,Yan, XZ,et al. Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,313(1):129-134.
APA Yao, YN,Zhang, QS,Yan, XZ,Zhu, G,&Wang, ED.(2004).Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,313(1),129-134.
MLA Yao, YN,et al."Escherichia coli tRNA(4)(Arg)(UCU) induces a constrained conformation of the crucial Omega-loop of arginyl-tRNA synthetase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 313.1(2004):129-134.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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