Expression of hepatitis C virus E2 ectodomain in E-coli and its application in the detection of anti-E2 antibodies in human sera
文献类型：期刊论文
| 作者 | Liu, J; Zhang, XX; Zhang, SY; Lu, M; Kong, YY; Wang, Y; Li, GD |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:1页码:57-63 |
| 关键词 | hepatitis C virus envelope protein E2 expression and purification Escherichia coli |
| 通讯作者 | Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,wangyuan@server.shcnc.ac.cn |
| 英文摘要 | The second envelope glycoprotein (E2) of hepatitis C virus has been shown to bind human target cells and has become a major target for the development of anti-HCV vaccines. Anti-E2 antibodies have been suggested to be of clinical significance in diagnosis, treatment and prognosis of hepatitis C. However, large-scale expression and purification of E2 proteins in mammalian cells is difficult. As an alternative, E2 fragment (aa 385-730) with a four-amino-acid mutation (aa 568-571 PCNI to RVTS) was expressed as hexa-histidine-tagged full length protein [E2N730(m)] in E. coli and purified to over 85% purity. Purified E2N730(m) was specifically recognized by homologous hepatitis C patient serum in Western blot, suggesting that it displayed E2-specific antigenicity. Rabbit antiserum raised against E2N730(m) recognized E2 glycoproteins expressed in mammalian cells in Western blot. Purified E2N730(in) was used to detect anti-E2 antibodies in human sera and showed better specificity and sensitivity than previously reported C-terminally truncated E2 fragment (aa 385-565). Association between anti-E2 antibodies in patient sera and HCV RNA status was also demonstrated using this E. coli-derived protein. E2N730(m) might serve as an inexpensive alternative to mammalian cell-expressed E2 proteins in clinical and research applications. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ENVELOPE PROTEINS ; LIVER-DISEASE ; HCV INFECTION ; VIRAL-RNA ; GLYCOPROTEIN ; CELLS ; IDENTIFICATION ; PREVALENCE ; RESPONSES ; PRODUCTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221073500012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2024]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, J,Zhang, XX,Zhang, SY,et al. Expression of hepatitis C virus E2 ectodomain in E-coli and its application in the detection of anti-E2 antibodies in human sera[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(1):57-63. |
| APA | Liu, J.,Zhang, XX.,Zhang, SY.,Lu, M.,Kong, YY.,...&Li, GD.(2004).Expression of hepatitis C virus E2 ectodomain in E-coli and its application in the detection of anti-E2 antibodies in human sera.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(1),57-63. |
| MLA | Liu, J,et al."Expression of hepatitis C virus E2 ectodomain in E-coli and its application in the detection of anti-E2 antibodies in human sera".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.1(2004):57-63. |
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