Identification of glutamate receptors and transporters in mouse and human sperm
文献类型：期刊论文
| 作者 | Hu, JH; Yang, N; Ma, YH; Jiang, J; Zhang, JF; Fei, J; Guo, LH |
| 刊名 | JOURNAL OF ANDROLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 25期号:1页码:140-146 |
| 关键词 | uptake immunocytochemistry RT-PCR inhibitor |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jfei@sibs.ac.cn ; mhzhang@sunm.shcnc.ac.cn |
| 英文摘要 | gamma-Aminobutyric acid (GABA) and glutamate (Glu) are considered as the predominant inhibitory and excitatory neurotransmitters in mammalian central nervous systems (CNS), respectively. The presence of the GABA system and metabotropic glutamate receptors in sperm prompted us to explore the existence of ionotropic glutamate receptors and glutamate transporters in sperm. Immunofluorescent analysis was used to investigate the existence and location of glutamate, glutamate receptor (NR2B), and glutamate transporter (GLT1) in mouse and human sperm. Our present results showed that NR2B was located in the midpiece of sperm, whereas GLT1 mainly existed in the head. Moreover, glutamate uptake activity was detected in mouse sperm and it could be blocked by dihydrokainic acid (DHK, GLT1-selective inhibitor) and DL-threo-betahydroxyaspartic acid (THA, nonselective inhibitor). In addition, reverse transcription-polymerase chain reaction technique and sequencing analysis revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2B, GluR6, and KA2) existed in mouse sperm as well as in human sperm. The present findings are the first direct evidence for the existence of ionotropic glutamate receptors and glutamate transporters in sperm. It also indicates that, in sperm, glutamate receptors and transporters might have functions other than neurotransmission. |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Andrology |
| 关键词[WOS] | GAMMA-AMINOBUTYRIC-ACID ; RAT TESTIS ; EXPRESSION ; EPIDIDYMIS ; BRAIN ; GABA ; SPERMATOZOA ; SUBUNITS ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220641300021 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2027]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, JH,Yang, N,Ma, YH,et al. Identification of glutamate receptors and transporters in mouse and human sperm[J]. JOURNAL OF ANDROLOGY,2004,25(1):140-146. |
| APA | Hu, JH.,Yang, N.,Ma, YH.,Jiang, J.,Zhang, JF.,...&Guo, LH.(2004).Identification of glutamate receptors and transporters in mouse and human sperm.JOURNAL OF ANDROLOGY,25(1),140-146. |
| MLA | Hu, JH,et al."Identification of glutamate receptors and transporters in mouse and human sperm".JOURNAL OF ANDROLOGY 25.1(2004):140-146. |
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