Co-treatment with ethanol enhances the toxicity of 6-hydroxydopamine
文献类型：期刊论文
| 作者 | Ming, Z; Lu, ZS; Gu, JF; Sun, LY; Liu, XY |
| 刊名 | NEUROSCIENCE LETTERS
 |
| 出版日期 | 2004 |
| 卷号 | 367期号:2页码:250-253 |
| 关键词 | ethanol 6-OHDA PC12 cell SK-N-SH cell degeneration |
| 通讯作者 | Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xyliu@sibs.ac.cn |
| 英文摘要 | 6-Hydroxydopamine (6-OHDA) is a widely used neural toxin in the pathogenesis research of Parkinson's disease (PD). In this work, we have studied the effect of ethanol on the toxicity of 6-OHDA on PC12 cell and SK-N-SH cell. Ethanol alone had little toxicity to these cells. However, if using 40 muM 6-OHDA along with 400 mM ethanol on PC12 cell or SK-N-SH cell for 24 h, there was much more cell loss than using 40 muM 6-OHDA alone when detected by 3-(4,5-dimethylthiazal-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay or flow cytometric assay. The toxicity of 6-OHDA was enhanced only if using at least 200 mM ethanol, and the cell loss was increased with the increase of ethanol concentration. We had also found that ethanol could enhance the toxicity of 6-OHDA only when using ethanol and 6-OHDA at the same time, ethanol treatment either before or after 6-OHDA treatment did not show such effect. This effect of ethanol suggests that ethanol may contribute to the degeneration of dopaminergic cells. (C) 2004 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | SK-N-SH ; PROTEIN-KINASE ; PC12 CELLS ; COMPLEX-I ; DOPAMINE ; DEGENERATION ; INHIBITION ; APOPTOSIS ; MECHANISM ; INCREASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000223783200027 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2032]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ming, Z,Lu, ZS,Gu, JF,et al. Co-treatment with ethanol enhances the toxicity of 6-hydroxydopamine[J]. NEUROSCIENCE LETTERS,2004,367(2):250-253. |
| APA | Ming, Z,Lu, ZS,Gu, JF,Sun, LY,&Liu, XY.(2004).Co-treatment with ethanol enhances the toxicity of 6-hydroxydopamine.NEUROSCIENCE LETTERS,367(2),250-253. |
| MLA | Ming, Z,et al."Co-treatment with ethanol enhances the toxicity of 6-hydroxydopamine".NEUROSCIENCE LETTERS 367.2(2004):250-253. |
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