Expression of ricin A chain and ricin A chain-KDEL in Escherichia coli
文献类型：期刊论文
| 作者 | Zhan, JB; Chen, YD; Wang, KY; Zheng, S |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2004 |
| 卷号 | 34期号:2页码:197-201 |
| 关键词 | ricin A chain endoplasmic reticulum retention signal |
| 通讯作者 | Zhan, JB (reprint author), Zhejiang Univ, Sch Med, Dept Biochem, 353 Yan An Rd, Hangzhou 310006, Peoples R China.,jzhan2k@cmm.zju.edu.cn |
| 英文摘要 | Ricin and its A chains can be used to conjugate with monoclonal antibodies to prepare immunotoxins. Ricin A chain (RTA) and its modification RTA-KDEL (ER-retrieval signal) were expressed with the pKK223.3 system in Escherichia coli under control of a tac promoter. The recombinant proteins can be purified by one-step affinity chromatography on a column of Blue-Sepharose 6B. The toxicities of RTA and its mutant RTA-KDEL were evaluated by the MTT assay in HeLa, MCF, and ECV-304 cells following fluid-phase endocytosis. RTA-KDEL was somewhat more cytotoxic than RTA itself in the different cell lines. The results suggest that rRTA-KDEL may be useful for the synthesis of more potent immunotoxins. (C) 2003 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | PSEUDOMONAS EXOTOXIN ; IMMUNOTOXINS ; CYTOTOXICITY ; CELLS ; SEQUENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220233400005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2036]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhan, JB,Chen, YD,Wang, KY,et al. Expression of ricin A chain and ricin A chain-KDEL in Escherichia coli[J]. PROTEIN EXPRESSION AND PURIFICATION,2004,34(2):197-201. |
| APA | Zhan, JB,Chen, YD,Wang, KY,&Zheng, S.(2004).Expression of ricin A chain and ricin A chain-KDEL in Escherichia coli.PROTEIN EXPRESSION AND PURIFICATION,34(2),197-201. |
| MLA | Zhan, JB,et al."Expression of ricin A chain and ricin A chain-KDEL in Escherichia coli".PROTEIN EXPRESSION AND PURIFICATION 34.2(2004):197-201. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。