Freeze-drying of mononuclear cells and whole blood of human cord blood
文献类型：期刊论文
| 作者 | Xiao, HH; Hua, TC; Li, J; Gu, XL; Wang, X; Wu, ZJ; Meng, LR; Gao, QR; Chen, J; Gong, ZP |
| 刊名 | CRYOLETTERS
 |
| 出版日期 | 2004 |
| 卷号 | 25期号:2页码:111-120 |
| 关键词 | freeze-drying human cord blood MNC lyoprotectant CD34(+) cell |
| 通讯作者 | Hua, TC (reprint author), Shanghai Univ Sci & Technol, Inst Cryomed & Food Refrigerat, No 516 Jun Gong Rd, Shanghai 200093, Peoples R China.,tchua@sh163.net |
| 英文摘要 | The research on haematopoietic stem cells of human cord blood has become more important recently. People have concentrated on the preservation of cord blood stem cells. At present, cord blood can be preserved at ultra-low temperatures. In this study, we try to preserve cord blood and its constituents by freeze-drying. The experiments on both the mononuclear cell content and the whole blood of human cord blood were carried out respectively. The samples were frozen firstly by different cooling protocols in the presence of PVP, sucrose, and mannitol. Afterwards, they were vacuum-dried at a selected shelf temperature of -30degreesC for the main drying stage, and then vacuum-dried at 15degreesC for the second drying stage. The entire time of the freeze drying was 52 hours. Samples were stored at room temperature for 2 days prior to evaluation. Subsequently, the dried samples were suspended in an isotonic phosphate-buffered saline solution. The recovery of the cells were tested by a haemacytometer, and the highest cell numerical count recovery of MNC was 75.0 +/- 4.1%(P<0.01), obtained in the protocol of 40% VP + 20% Sucrose + 10% Mannitol. The viability of the nucleated cells measured by PI staining and the ratio of the number of CD34(+) to the number of lymphocytes (by the FITC anti-human CD34(+) conjugated antibody method) were measured using a flow cytometer (FCM). The protocol of 40% PVP + 20% Sucrose + 10% fetal bovine serum had the highest viability of 98.57 &PLUSMN; 0.68%(P<0.01). The highest ratio of CD34(+) to lymphocytes was 1.17%, and the highest recovery of C1334(+) was 68.42 +/- 39.5% (P<0.05). Comparing the results of the lyophilized MNC subfraction with that of the whole blood, the lyophilization of the isolated MNC was more successful than that of whole blood. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics; Physiology |
| 类目[WOS] | Biology ; Physiology |
| 关键词[WOS] | UNRELATED BONE-MARROW ; COOLING RATE ; CRYOPRESERVATION ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221643700004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2037]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xiao, HH,Hua, TC,Li, J,et al. Freeze-drying of mononuclear cells and whole blood of human cord blood[J]. CRYOLETTERS,2004,25(2):111-120. |
| APA | Xiao, HH.,Hua, TC.,Li, J.,Gu, XL.,Wang, X.,...&Gong, ZP.(2004).Freeze-drying of mononuclear cells and whole blood of human cord blood.CRYOLETTERS,25(2),111-120. |
| MLA | Xiao, HH,et al."Freeze-drying of mononuclear cells and whole blood of human cord blood".CRYOLETTERS 25.2(2004):111-120. |
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