Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells
文献类型：期刊论文
| 作者 | Gong, YQ; Fan, Y; Liu, L; Wu, DZ; Chang, ZL; Wang, ZT |
| 刊名 | IN VIVO
 |
| 出版日期 | 2004 |
| 卷号 | 18期号:2页码:223-228 |
| 关键词 | erianin endothelial cells acidification rate metabolism JNK/SAPK |
| 通讯作者 | Wang, ZT (reprint author), Shanghai Univ Trad Chinese Med, Inst Chinese Mat Med, 1200 Cailun Rd,Zhangijang Hi Tech Pk, Shanghai 201203, Peoples R China.,wangzht@shutcm.edu.cn |
| 英文摘要 | Background Erianin is a natural product derived from Dendrobium chrysotoxum, with promising antitumor activity. Materials and Methods: To evaluate the metabolic effect of erianin, a cytosensor assay for acidification rate, MTT assay, measurement of lactate, glucose and ATP were performed in human umbilical vein endothelial cells (HUVECs) exposed to 1100 nM erianin. JNK/SAPK activity was detected by Western blot. Results: Twelve- or 24- hour incubation with erianin induced a dose-dependent metabolic inhibition, as indicated by reduced acidification rate and cell viability, with an endothelium-selectivity. Erianin caused decreases in lactate production, glucose consumption and intracellular ATP level. Pretreatment with the JNK/SAPK inhibitor SP600125 significantly abolished these inhibitory responses, and especially restored the erianin-induced decreases in ATP and the erianin-induced phosphorylation of JNK/SAPK with dose- and time- dependence. Conclusion: Erianin inhibited endothelial metabolism in a JNK/SAPK-dependent manner. This mechanism may be involved in the potential antitumor and antiangiogenic actions of erianin. |
| 学科主题 | Research & Experimental Medicine |
| 类目[WOS] | Medicine, Research & Experimental |
| 关键词[WOS] | HUMAN TUMOR-CELLS ; IN-VITRO ; APOPTOSIS ; ACTIVATION ; HYPOXIA ; PATHWAY ; JNK ; TECHNOLOGY ; BIBENZYL ; STRESS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220784000016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2038]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gong, YQ,Fan, Y,Liu, L,et al. Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells[J]. IN VIVO,2004,18(2):223-228. |
| APA | Gong, YQ,Fan, Y,Liu, L,Wu, DZ,Chang, ZL,&Wang, ZT.(2004).Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells.IN VIVO,18(2),223-228. |
| MLA | Gong, YQ,et al."Erianin induces a JNK/SAPK-dependent metabolic inhibition in human umbilical vein endothelial cells".IN VIVO 18.2(2004):223-228. |
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