Preparation and primary application of monoclonal antibodies against a novel ribosome-inactivating protein moschatin from pumpkin seeds
文献类型：期刊论文
| 作者 | Xia, HC; Hu, WG; Yang, XX; Li, F; Zhang, ZC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2004 |
| 卷号 | 36期号:2页码:105-110 |
| 关键词 | moschatin monoclonal antibody affinity chromatography Western blot |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Prote, Shanghai 200031, Peoples R China.,zhangzc@summ.shcnc.ac.cn |
| 英文摘要 | Plant ribosome-inactivating proteins (RIPs) have multiple biological functions, and have been widely used in the studies on biomedical and agronomic applications. Moschatin is a novel single-chain RIP recently purified from pumpkin seeds, and it has been successfully applied to construct the immunotoxin that can selectively kill the cultured human melanoma cells. Six stable strains of hybridomas (2H8, 4A8, 5136, 6F8, 4H10 and 6C2) that can secrete high specific monoclonal antibodies against Moschatin have been successfully prepared using hybridoma technique. The isotypes of these monoclonal antibodies are IgG(1), IgGj(1), IgG(1), IgG(1), IgG(2a), and IgM. Their affinity constants were determined to be 1.42 x 10(8), 2.71 x 10(8), 8.72 x 10(7), 2.06 x 10(8), 1.36 x 10(8) and 1.51 x 10(8) M-1 in a sequent order, measured by non-competitive ELISA. The monoclonal antibody 4A8 has been used to detect Moschatin in Western blot. An immunoaffinity gel, which consisted of a monoclonal antibody 4H10 and Sepharose 4B, was prepared and used to purify Moschatin from pumpkin seeds crude extract. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | N-GLYCOSIDASE ACTIVITY ; EUKARYOTIC RIBOSOMES ; ANTIFUNGAL ACTIVITY ; MECHANISM ; PLANTS ; TRICHOSANTHIN ; APOPTOSIS ; INDUCTION ; LUFFIN ; CHAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000221073600004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2040]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xia, HC,Hu, WG,Yang, XX,et al. Preparation and primary application of monoclonal antibodies against a novel ribosome-inactivating protein moschatin from pumpkin seeds[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2004,36(2):105-110. |
| APA | Xia, HC,Hu, WG,Yang, XX,Li, F,&Zhang, ZC.(2004).Preparation and primary application of monoclonal antibodies against a novel ribosome-inactivating protein moschatin from pumpkin seeds.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,36(2),105-110. |
| MLA | Xia, HC,et al."Preparation and primary application of monoclonal antibodies against a novel ribosome-inactivating protein moschatin from pumpkin seeds".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 36.2(2004):105-110. |
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