Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication
文献类型：期刊论文
| 作者 | Ren, Y; Wu, JR |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2004 |
| 卷号 | 14期号:3页码:227-233 |
| 关键词 | intra-S-phase checkpoint DNA replication DNA damage tripchlorolide |
| 通讯作者 | Wu, JR (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,wujr@sibs.ac.cn |
| 英文摘要 | DNA replication is tightly regulated during the S phase of the cell cycle, and the activation of the intra-S-phase checkpoint due to DNA damage usually results in arrest of DNA synthesis. However, the molecular details about the correlation between the checkpoint and regulation of DNA replication are still unclear. To investigate the connections between DNA replication and DNA damage checkpoint, a DNA-damage reagent, tripchlorolide, was applied to CHO (Chinese ovary hamster) cells at early- or middle-stages of the S phase. The early-S-phase treatment with TC significantly delayed the progression of the S phase and caused the phosphorylation of the Chk1 checkpoint protein, whereas the middle-S-phase treatment only slightly slowed down the progression of the S phase. Furthermore, the analysis of DNA replication patterns revealed that replication pattern 11 was greatly prolonged in the cells treated with the drug during the early-S phase, whereas the late-replication patterns of these cells were hardly detected, suggesting that the activation of the intra-S-phase checkpoint inhibits the late-origin firing of DNA replication. We conclude that cells at different stages of the S phase are differentially sensitive to the DNA-damage reagent, and the activation of the intra-S-phase checkpoint blocks the DNA replication progression in the late stage of S phase. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | DOUBLE-STRAND BREAKS ; SACCHAROMYCES-CEREVISIAE ; DAMAGE CHECKPOINT ; HISTONE H2AX ; ORIGINS ; CHK1 ; ATM ; PROGRESSION ; CELLS ; PHOSPHORYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222454200006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2053]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ren, Y,Wu, JR. Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication[J]. CELL RESEARCH,2004,14(3):227-233. |
| APA | Ren, Y,&Wu, JR.(2004).Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication.CELL RESEARCH,14(3),227-233. |
| MLA | Ren, Y,et al."Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication".CELL RESEARCH 14.3(2004):227-233. |
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