Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis
文献类型：期刊论文
| 作者 | Zeng, R; Ruan, HQ; Jiang, XS; Zhou, H; Shi, L; Zhang, L; Sheng, QH; Tu, Q; Xia, QC; Wu Jr |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2004 |
| 卷号 | 3期号:3页码:549-555 |
| 关键词 | coronavirus proteome mass spectrometry glycosylation phosphorylation |
| 通讯作者 | Zeng, R (reprint author), CAS, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn ; wujr@sibs.ac.cn |
| 英文摘要 | The proteomes of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and its infected Vero E6 cells were detected in the present study. The cytosol and nucleus fractions of virus infected cells as well as the crude virions were analyzed either by one-dimensional electrophoresis followed by ESI-MS/MS identification or by shotgun strategy with two-dimensional liquid chromatography-ESI-MS/MS. For the first time, all of the four predicted structural proteins of SARS-CoV were identified, including S (Spike), M (Membrane), N (Nucleocapsid), and E (Envolope) proteins. In addition, a novel phosphorylated site of M protein was observed. The combination of these gel-base and non-gel methods provides fast and complimentary approaches to SARS-CoV proteome and can be widely used in the analysis of other viruses. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; MOUSE HEPATITIS-VIRUS ; ENVELOPE PROTEIN ; MEMBRANE-PROTEIN ; CELL-DIVISION ; HOST-CELL ; REPLICATION ; GENOME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000222075500023 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2055]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zeng, R,Ruan, HQ,Jiang, XS,et al. Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis[J]. JOURNAL OF PROTEOME RESEARCH,2004,3(3):549-555. |
| APA | Zeng, R.,Ruan, HQ.,Jiang, XS.,Zhou, H.,Shi, L.,...&Wu Jr.(2004).Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis.JOURNAL OF PROTEOME RESEARCH,3(3),549-555. |
| MLA | Zeng, R,et al."Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis".JOURNAL OF PROTEOME RESEARCH 3.3(2004):549-555. |
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